Human intrahepatic biliary epithelial cells engulf blebs from their apoptotic peers

G. H. Rong, G. X. Yang, Y. Ando, Weici Zhang, Xiaosong He, Patrick S Leung, R. L. Coppel, A. A. Ansari, R. Zhong, M. Eric Gershwin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Summary: The phagocytic clearance of apoptotic cells is critical for tissue homeostasis; a number of non-professional phagocytic cells, including epithelial cells, can both take up and process apoptotic bodies, including the release of anti-inflammatory mediators. These observations are particularly important in the case of human intrahepatic biliary cells (HiBEC), because such cells are themselves a target of destruction in primary biliary cirrhosis, the human autoimmune disease. To address the apoptotic ability of HiBECs, we have focused on their ability to phagocytize apoptotic blebs from autologous HiBECs. In this study we report that HiBEC cells demonstrate phagocytic function from autologous HiBEC peers accompanied by up-regulation of the chemokines CCL2 [monocyte chemotactic protein-1 (MCP-1)] and CXCL8 [interleukin (IL)-8]. In particular, HiBEC cells express the phagocytosis-related receptor phosphatidylserine receptors (PSR), implying that HiBECs function through the 'eat-me' signal phosphatidylserine expressed by apoptotic cells. Indeed, although HiBEC cells acquire antigen-presenting cell (APC) function, they do not change the expression of classic APC function surface markers after engulfment of blebs, both with and without the presence of Toll-like receptor (TLR) stimulation. These results are important not only for understanding of the normal physiological function of HiBECs, but also explain the inflammatory potential and reduced clearance of HiBEC cells following the inflammatory cascade in primary biliary cirrhosis.

Original languageEnglish (US)
Pages (from-to)95-103
Number of pages9
JournalClinical and Experimental Immunology
Volume172
Issue number1
DOIs
StatePublished - Apr 2013

Fingerprint

Blister
Epithelial Cells
Biliary Liver Cirrhosis
Chemokine CCL2
Antigen-Presenting Cells
Phagocytes
Cytophagocytosis
Toll-Like Receptors
Phosphatidylserines
Interleukin-8
Autoimmune Diseases
Homeostasis
Anti-Inflammatory Agents
Up-Regulation

Keywords

  • Apoptosis
  • Chemokine
  • Intrahepatic biliary epithelial cells
  • Phagocytosis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Human intrahepatic biliary epithelial cells engulf blebs from their apoptotic peers. / Rong, G. H.; Yang, G. X.; Ando, Y.; Zhang, Weici; He, Xiaosong; Leung, Patrick S; Coppel, R. L.; Ansari, A. A.; Zhong, R.; Gershwin, M. Eric.

In: Clinical and Experimental Immunology, Vol. 172, No. 1, 04.2013, p. 95-103.

Research output: Contribution to journalArticle

Rong, G. H. ; Yang, G. X. ; Ando, Y. ; Zhang, Weici ; He, Xiaosong ; Leung, Patrick S ; Coppel, R. L. ; Ansari, A. A. ; Zhong, R. ; Gershwin, M. Eric. / Human intrahepatic biliary epithelial cells engulf blebs from their apoptotic peers. In: Clinical and Experimental Immunology. 2013 ; Vol. 172, No. 1. pp. 95-103.
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