Human insulin-like growth factor I exerts hematopoietic growth-promoting effects after in vivo admimistration

G. Tsarfaty, D. L. Longo, William J Murphy

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Recombinant human insulin-like growth factor I (rhIGF-I) was administered to mice to determine its effect on hematopoiesis. Mice given intraperitoneal (IF) injections of 5 μg rhIGF-I for 7 days exhibited a significant increase in bone marrow (BM) hematopoietic progenitor cells, suggesting that rhIGF-I acts as a hematopoietic growth factor. Treatment with rhIGF-I also resulted in increases in erythroid precursor cells. Mice were then placed on azidothymidine (AZT), which produces significant myelotoxic effects. Splenic and BM progenitor cell content and hematocrit values were all significantly increased if rhIGF-I (5 μg injected IP every day for 28 days) was concurrently administered with AZT. Additionally, when mice were initially myelosuppressed by several weeks of AZT treatment, the subsequent administration of rhIGF-I resulted in an increase in hematopoietic progenitor cell content. No significant pathologic effects or weight changes were observed in mice receiving repeated injections of rhIGF-I at this dose and schedule. Thus, rhLGF-I exerts hematopoietic growth-promoting effects in vivo and may be of potential clinical use in promoting hematopoiesis in the face of myelotoxic therapy.

Original languageEnglish (US)
Pages (from-to)1273-1277
Number of pages5
JournalExperimental Hematology
Volume22
Issue number13
StatePublished - 1994
Externally publishedYes

Keywords

  • Azidothymidine
  • Growth hormone
  • Hematopoiesis
  • Insulin-like growht factor I
  • Neuroendocrine

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Fingerprint Dive into the research topics of 'Human insulin-like growth factor I exerts hematopoietic growth-promoting effects after in vivo admimistration'. Together they form a unique fingerprint.

  • Cite this