Human galectin-3 is a novel chemoattractant for monocytes and macrophages

H. Sano, D. K. Hsu, L. Yu, J. R. Apgar, I. Kuwabara, T. Yamanaka, M. Hirashima, Fu-Tong Liu

Research output: Contribution to journalArticle

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Abstract

Galectin-3 is a β-galactoside-binding protein implicated in diverse biological processes. We found that galectin-3 induced human monocyte migration in vitro in a dose-dependent manner, and it was chemotactic at high concentrations (1.0 μM) but chemokinetic at low concentrations (10-100 nM). Galectin-3-induced monocyte migration was inhibited by its specific mAb and was blocked by lactose and a C-terminal domain fragment of the protein, indicating that both the N-terminal and C-terminal domains of galectin-3 are involved in this activity. Pertussis toxin (PTX) almost completely blocked monocyte migration induced by high concentrations of galectin-3. Galectin-3 caused a Ca2+ influx in monocytes at high, but not low, concentrations, and both lactose and PTX inhibited this response. There was no cross- desensitization between galectin-3 and any of the monocyte-reactive chemokines examined, including monocyte chemotactic protein-1, macrophage inflammatory protein-1α, and stromal cell-derived factor-1α. Cultured human macrophages and alveolar macrophages also migrated toward galectin-3, but not monocyte chemotactic protein-1. Finally, galectin-3 was found to cause monocyte accumulation in vivo in mouse air pouches. These results indicate that galectin-3 is a novel chemoattractant for monocytes and macrophages and suggest that the effect is mediated at least in part through a PTX-sensitive (G protein-coupled) pathway.

Original languageEnglish (US)
Pages (from-to)2156-2164
Number of pages9
JournalJournal of Immunology
Volume165
Issue number4
StatePublished - Aug 15 2000

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Galectin 3
Chemotactic Factors
Monocytes
Macrophages
Pertussis Toxin
Chemokine CCL2
Lactose
Biological Phenomena
Macrophage Inflammatory Proteins
human galectin-3
Chemokine CXCL12
Galactosides
Alveolar Macrophages
Chemokines
GTP-Binding Proteins
Carrier Proteins
Air

ASJC Scopus subject areas

  • Immunology

Cite this

Sano, H., Hsu, D. K., Yu, L., Apgar, J. R., Kuwabara, I., Yamanaka, T., ... Liu, F-T. (2000). Human galectin-3 is a novel chemoattractant for monocytes and macrophages. Journal of Immunology, 165(4), 2156-2164.

Human galectin-3 is a novel chemoattractant for monocytes and macrophages. / Sano, H.; Hsu, D. K.; Yu, L.; Apgar, J. R.; Kuwabara, I.; Yamanaka, T.; Hirashima, M.; Liu, Fu-Tong.

In: Journal of Immunology, Vol. 165, No. 4, 15.08.2000, p. 2156-2164.

Research output: Contribution to journalArticle

Sano, H, Hsu, DK, Yu, L, Apgar, JR, Kuwabara, I, Yamanaka, T, Hirashima, M & Liu, F-T 2000, 'Human galectin-3 is a novel chemoattractant for monocytes and macrophages', Journal of Immunology, vol. 165, no. 4, pp. 2156-2164.
Sano H, Hsu DK, Yu L, Apgar JR, Kuwabara I, Yamanaka T et al. Human galectin-3 is a novel chemoattractant for monocytes and macrophages. Journal of Immunology. 2000 Aug 15;165(4):2156-2164.
Sano, H. ; Hsu, D. K. ; Yu, L. ; Apgar, J. R. ; Kuwabara, I. ; Yamanaka, T. ; Hirashima, M. ; Liu, Fu-Tong. / Human galectin-3 is a novel chemoattractant for monocytes and macrophages. In: Journal of Immunology. 2000 ; Vol. 165, No. 4. pp. 2156-2164.
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