Abstract
Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.
Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.
Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.
Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 31827-31836 |
| Number of pages | 10 |
| Journal | Journal of Biological Chemistry |
| Volume | 289 |
| Issue number | 46 |
| DOIs | |
| State | Published - Nov 14 2014 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
- Medicine(all)
Cite this
Sokabe, M., & Fraser, C. S. (2014). Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex. Journal of Biological Chemistry, 289(46), 31827-31836. https://doi.org/10.1074/jbc.M114.602870