Abstract
Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.
Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.
Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.
Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 31827-31836 |
| Number of pages | 10 |
| Journal | Journal of Biological Chemistry |
| Volume | 289 |
| Issue number | 46 |
| DOIs | |
| State | Published - Nov 14 2014 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
- Medicine(all)
Cite this
Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex. / Sokabe, Masaaki; Fraser, Christopher S.
In: Journal of Biological Chemistry, Vol. 289, No. 46, 14.11.2014, p. 31827-31836.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex
AU - Sokabe, Masaaki
AU - Fraser, Christopher S.
PY - 2014/11/14
Y1 - 2014/11/14
N2 - Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.
AB - Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.
UR - http://www.scopus.com/inward/record.url?scp=84911192188&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84911192188&partnerID=8YFLogxK
U2 - 10.1074/jbc.M114.602870
DO - 10.1074/jbc.M114.602870
M3 - Article
C2 - 25246524
AN - SCOPUS:84911192188
VL - 289
SP - 31827
EP - 31836
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 46
ER -