Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex

Masaaki Sokabe, Christopher S. Fraser

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.

Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.

Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.

Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.

Original languageEnglish (US)
Pages (from-to)31827-31836
Number of pages10
JournalJournal of Biological Chemistry
Volume289
Issue number46
DOIs
StatePublished - Nov 14 2014

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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