Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex

Masaaki Sokabe; Christopher S. Fraser

doi:10.1074/jbc.M114.602870

Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex

Masaaki Sokabe, Christopher S. Fraser

Molecular and Cellular Biology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.

Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.

Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.

Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.

Original language	English (US)
Pages (from-to)	31827-31836
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	289
Issue number	46
DOIs	https://doi.org/10.1074/jbc.M114.602870
State	Published - Nov 14 2014

ASJC Scopus subject areas

Biochemistry
Cell Biology
Molecular Biology
Medicine(all)

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abstract = "Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.",

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AB - Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation.Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components.Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC.Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.

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Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex

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