Human erythropoietin gene: High level expression in stably transfected mammalian cells and chromosome localization

Jerry S Powell, K. L. Berkner, R. V. Lebo, J. W. Adamson

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The glycoprotein hormone erythropoietin plays a major role in regulating erythropoiesis and deficiencies of erythropoietin result in anemia. Detailed studies of the hormone and attempts at replacement therapy have been difficult due to the scarcity of purified material. We used a cloned human erythropoietin gene to develop stably transfected mammalian cell lines that secrete large amounts of the hormone with potent biological activity. These cell lines were produced by cotransfection of mammalian cells with a plasmid containing a selectable marker and plasmid constructions containing a cloned human erythropoietin gene inserted next to a strong promoter. The protein secreted by these cells stimulated the proliferation and differentiation of erythroid progenitor cells and, with increased selection, several of these cell lines secrete up to 80 mg of the protein per liter of supernatant. Hybridization analysis of DNA from human chromosomes isolated by high resolution dual laser sorting provides evidence that the gene for human erythropoietin is located on human chromosome 7.

Original languageEnglish (US)
Pages (from-to)6465-6469
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number17
StatePublished - 1986
Externally publishedYes

Fingerprint

Mammalian Chromosomes
Erythropoietin
Genes
Human Chromosomes
Hormones
Cell Line
Plasmids
Erythroid Precursor Cells
Chromosomes, Human, Pair 7
Erythropoiesis
Anemia
Cell Differentiation
Glycoproteins
Proteins
Lasers
Cell Proliferation
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Human erythropoietin gene : High level expression in stably transfected mammalian cells and chromosome localization. / Powell, Jerry S; Berkner, K. L.; Lebo, R. V.; Adamson, J. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 17, 1986, p. 6465-6469.

Research output: Contribution to journalArticle

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