Human dopa decarboxylase: Localization to human chromosome 7p11 and characterization of hepatic cDNAs

Lisa J. Scherer, John Douglas Mcpherson, John J. Wasmuth, J. Lawrence Marsh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have cloned full-length DDC cDNAs from a human hepatoma cDNA library [DDC; dopa decarboxylase; aromatic-L-amino acid decarboxylase, EC 4.1.1.28]. The protein encoded by hepatoma cells is the same as that encoded by adrenal chromaffin derived pheochromocytoma cells, despite reported differences in biochemical properties. We have confirmed the location of the DDC gene to chromosome 7 using a new panel of somatic cell hybrids, and we have localized the gene to band p11 on chromosome 7 by fluorescent in situ hybridization. The human gene retains 65% amino acid identity with Drosophila DDC (Accession No. X04426) and considerable structural similarity with other enzymes (F.R. Jackson, 1990, J. Mol. Evol. 31: 325-329, and references therein).

Original languageEnglish (US)
Pages (from-to)469-471
Number of pages3
JournalGenomics
Volume13
Issue number2
DOIs
StatePublished - 1992

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Dopa Decarboxylase
Human Chromosomes
Chromosomes, Human, Pair 7
Complementary DNA
Hepatocellular Carcinoma
Liver
Aromatic-L-Amino-Acid Decarboxylases
Genes
Hybrid Cells
Pheochromocytoma
Fluorescence In Situ Hybridization
Gene Library
Drosophila
Amino Acids
Enzymes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Human dopa decarboxylase : Localization to human chromosome 7p11 and characterization of hepatic cDNAs. / Scherer, Lisa J.; Mcpherson, John Douglas; Wasmuth, John J.; Marsh, J. Lawrence.

In: Genomics, Vol. 13, No. 2, 1992, p. 469-471.

Research output: Contribution to journalArticle

Scherer, Lisa J. ; Mcpherson, John Douglas ; Wasmuth, John J. ; Marsh, J. Lawrence. / Human dopa decarboxylase : Localization to human chromosome 7p11 and characterization of hepatic cDNAs. In: Genomics. 1992 ; Vol. 13, No. 2. pp. 469-471.
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