Human cytomegalovirus strain toledo lacks a virus-encoded tropism factor required for infection of aortic endothelial cells

C. Bolovan-Fritts, Jean A Wiedeman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV) strains were investigated to identify those with altered tropism for endothelial cells. In viral replication kinetics analysis, HCMV strain Toledo replicated poorly in aortic endothelial cells (AECs), and the virus count was reduced by 2-3 log units, in comparison with strain AD169. Virus entry at the cell surface for each strain was equivalent. However, immunofluorescence studies revealed a lack of immediate early viral antigen 72 expression, and direct blot hybridization failed to detect viral genomes in the nucleus of Toledo-infected AECs. Complementation assays were done to determine whether endothelial cell infectivity was dependent on a virus-encoded tropism factor. Pseudotype virus in endothelial cell cultures indicated that AD169 could provide trans factors to rescue Toledo during infection of endothelial cells. Collectively, these results show that a viral function provides an endothelial cell tropism factor for HCMV and plays a role during postentry infection events.

Original languageEnglish (US)
Pages (from-to)1252-1261
Number of pages10
JournalJournal of Infectious Diseases
Volume184
Issue number10
DOIs
StatePublished - Nov 15 2001

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Tropism
Cytomegalovirus
Endothelial Cells
Viruses
Infection
Virus Internalization
Viral Antigens
Viral Genome
Fluorescent Antibody Technique
Cell Culture Techniques
Cell Count

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Human cytomegalovirus strain toledo lacks a virus-encoded tropism factor required for infection of aortic endothelial cells. / Bolovan-Fritts, C.; Wiedeman, Jean A.

In: Journal of Infectious Diseases, Vol. 184, No. 10, 15.11.2001, p. 1252-1261.

Research output: Contribution to journalArticle

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