Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease.

Gaosheng Huang, Qingguo Yan, Zhe Wang, Xiequn Chen, Xiaohui Zhang, Ying Guo, Jian-Jian Li

Research output: Contribution to journalArticle

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Abstract

Although Epstein-Barr virus (EBV) infection has been implicated in the development of a subset of Hodgkin's disease, the etiology of EBV-negative Hodgkin's disease remains to be determined. We investigated the status of human cytomegalovirus (hCMV) infection in 42 cases of Hodgkin's disease in a Chinese population. Using a nested polymerase chain reaction assay, nine of the 42 cases (21.4%) were found to contain EBV DNA. While only one of the nine EBV-positive cases demonstrated amplifiable hCMV DNA, approximately 33.3% of the EBV-negative cases (11/33) showed molecular evidence of hCMV infection (8 mixed cellularity; 2 nodular sclerosis; and 1 lymphocyte depletion). All 17 lymph nodes with non-specific reactive changes were negative for hCMV DNA. The presence of hCMV in Reed-Sternberg cells and their variants was further confirmed by single-cell polymerase chain reaction, in situ hybridization and immunohistochemical analysis, which demonstrated ~50% amplified hCMV DNA. These results suggest for the first time that hCMV infection plays a potential role in signaling the cell transformation and pathogenesis of Hodgkin's disease, particularly in EBV-negative cases.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalInternational Journal of Oncology
Volume21
Issue number1
StatePublished - Jul 2002
Externally publishedYes

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Cytomegalovirus
Human Herpesvirus 4
Hodgkin Disease
Cytomegalovirus Infections
DNA
Lymphocyte Depletion
Reed-Sternberg Cells
Polymerase Chain Reaction
Epstein-Barr Virus Infections
Sclerosis
In Situ Hybridization
Lymph Nodes
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease. / Huang, Gaosheng; Yan, Qingguo; Wang, Zhe; Chen, Xiequn; Zhang, Xiaohui; Guo, Ying; Li, Jian-Jian.

In: International Journal of Oncology, Vol. 21, No. 1, 07.2002, p. 31-36.

Research output: Contribution to journalArticle

Huang, G, Yan, Q, Wang, Z, Chen, X, Zhang, X, Guo, Y & Li, J-J 2002, 'Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease.', International Journal of Oncology, vol. 21, no. 1, pp. 31-36.
Huang, Gaosheng ; Yan, Qingguo ; Wang, Zhe ; Chen, Xiequn ; Zhang, Xiaohui ; Guo, Ying ; Li, Jian-Jian. / Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease. In: International Journal of Oncology. 2002 ; Vol. 21, No. 1. pp. 31-36.
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