Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality

W. L William Chang, Nicole Baumgarth, Dong Yu, Peter A Barry

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Interleukin-10 (IL-10) suppresses the maturation and cytokine production of dendritic cells (DCs), key regulators of adaptive immunity, and prevents the activation and polarization of naïve T cells towards protective gamma interferon-producing effectors. We hypothesized that human cytomegalovirus (HCMV) utilizes its viral IL-10 homolog (cmvIL-10) to attenuate DC functionality, thereby subverting the efficient induction of antiviral immune responses. RNA and protein analyses demonstrated that the cmvIL-10 gene was expressed with late gene kinetics. Treatment of immature DCs (iDCs) with supernatant from HCMV-infected cultures inhibited both the lipopolysaccharide- induced DC maturation and proinflammatory cytoldne production. These inhibitory effects were specifically mediated through the IL-10 receptor and were not observed when DCs were treated with supernatant of cells infected with a cmvIL-10-knockout mutant. Incubation of iDCs with recombinant cmvIL-10 recapitulated the inhibition of maturation. Furthermore, cmvIL-10 had pronounced long-term effects on those DCs that could overcome this inhibition of maturation. It enhanced the migration of mature DCs (mDCs) towards the lymph node homing chemoldne but greatly reduced their cytokine production. The inability of mDCs to secrete IL-12 was maintained, even when they were restimulated by the activated T-cell signal CD40 ligand in the absence of cmvIL-10. Importantly, cmvIL-10 potentiates these anti-inflammatory effects, at least partially, by inducing endogenous cellular IL-10 expression in DCs. Collectively, we show that cmvIL-10 causes long-term functional alterations at all stages of DC activation.

Original languageEnglish (US)
Pages (from-to)8720-8731
Number of pages12
JournalJournal of Virology
Volume78
Issue number16
DOIs
StatePublished - Aug 2004

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Human herpesvirus 5
dendritic cells
interleukin-10
Cytomegalovirus
Interleukin-10
Dendritic Cells
Interleukin-10 Receptors
cytokines
T-lymphocytes
Cytokines
immatures
T-Lymphocytes
CD40 Ligand
knockout mutants
Adaptive Immunity
Interleukin-12
interleukin-12
interferon-gamma
anti-inflammatory activity
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. / Chang, W. L William; Baumgarth, Nicole; Yu, Dong; Barry, Peter A.

In: Journal of Virology, Vol. 78, No. 16, 08.2004, p. 8720-8731.

Research output: Contribution to journalArticle

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