Human Coxsackie adenovirus receptor (CAR) expression in transgenic mouse prostate tumors enhances adenoviral delivery of genes

Yunhua Bao, Weidan Peng, Amy Verbitsky, Jiping Chen, Lily Wu, Katherine A Rauen, Janet A. Sawicki

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

BACKGROUND. Transgenic mice that recapitulate the progression of human diseases are potentially useful models for testing the effectiveness of new therapeutic strategies. Their use in pre-clinical testing of adenovirally-delivered gene therapies, however, is limited because of restricted cell surface expression of Coxsackie adenovirus receptor (CAR) in mice. METHODS. To develop a more suitable transgenic mouse model for testing adenoviral-based gene therapies for prostate cancer, we generated prostate specific antigen/human CAR (PSA/hCAR) transgenic mice in which a chimeric enhancer/promoter sequence of the human PSA gene drives expression of a functional hCAR coding sequence. RESULTS. Expression of an adenovirally- delivered luciferase reporter gene in prostate tumor cells in bigenic mice (PSA/hCAR + TRAMP) was enhanced compared to the level in tumor cells lacking the PSA/hCAR transgene. CONCLUSIONS. Breeding PSA/hCAR mice to existing transgenic mouse models for prostate cancer (e.g., TRAMP) results in improved mouse models for testing adenovirally-delivered therapeutic genes.

Original languageEnglish (US)
Pages (from-to)401-407
Number of pages7
JournalProstate
Volume64
Issue number4
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

Keywords

  • Adenoviral vector
  • Coxsackie adenoviral receptor
  • Gene therapy
  • Prostate cancer
  • Transgenic mice

ASJC Scopus subject areas

  • Urology

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