Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction

Claus S. Sondergaard, David A. Hess, Dustin J. Maxwell, Carla Weinheimer, Ivana Rosová, Michael H. Creer, David Piwnica-Worms, Attila Kovacs, Lene Pedersen, Jan Nolta

Research output: Contribution to journalArticle

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Abstract

Human stem cells from adult sources have been shown to contribute to the regeneration of muscle, liver, heart, and vasculature. The mechanisms by which this is accomplished are, however, still not well understood. We tested the engraftment and regenerative potential of human umbilical cord blood-derived ALDHhiLin-, and ALDHloLin- cells following transplantation to NOD/SCID or NOD/SCID β2m null mice with experimentally induced acute myocardial infarction. We used combined nanoparticle labeling and whole organ fluorescent imaging to detect human cells in multiple organs 48 hours post transplantation. Engraftment and regenerative effects of cell treatment were assessed four weeks post transplantation. We found that ALDHhiLin- stem cells specifically located to the site of injury 48 hours post transplantation and engrafted the infarcted heart at higher frequencies than ALDHloLin- committed progenitor cells four weeks post transplantation. We found no donor derived cardiomyocytes and few endothelial cells of donor origin. Cell treatment was not associated with any detectable functional improvement at the four week endpoint. There was, however, a significant increase in vascular density in the central infarct zone of ALDHhiLin- cell-treated mice, as compared to PBS and ALDHloLin- cell-treated mice.Conclusions: Our data indicate that adult human stem cells do not become a significant part of the regenerating tissue, but rapidly home to and persist only temporarily at the site of hypoxic injury to exert trophic effects on tissue repair thereby enhancing vascular recovery.

Original languageEnglish (US)
Article number24
JournalJournal of Translational Medicine
Volume8
DOIs
StatePublished - Mar 9 2010

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Aldehyde Dehydrogenase
Stem cells
Fetal Blood
Blood Vessels
Blood
Myocardial Infarction
Transplantation
Adult Stem Cells
Tissue
Endothelial cells
Stem Cells
Liver
Labeling
Muscle
Repair
Cells
Liver Regeneration
Nanoparticles
Cell Transplantation
Wounds and Injuries

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction. / Sondergaard, Claus S.; Hess, David A.; Maxwell, Dustin J.; Weinheimer, Carla; Rosová, Ivana; Creer, Michael H.; Piwnica-Worms, David; Kovacs, Attila; Pedersen, Lene; Nolta, Jan.

In: Journal of Translational Medicine, Vol. 8, 24, 09.03.2010.

Research output: Contribution to journalArticle

Sondergaard, Claus S. ; Hess, David A. ; Maxwell, Dustin J. ; Weinheimer, Carla ; Rosová, Ivana ; Creer, Michael H. ; Piwnica-Worms, David ; Kovacs, Attila ; Pedersen, Lene ; Nolta, Jan. / Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction. In: Journal of Translational Medicine. 2010 ; Vol. 8.
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AU - Creer, Michael H.

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