Human chromosome-specific cDNA libraries: New tools for gene identification and genome annotation

R. G. Del Mastro, L. Wang, A. D. Simmons, T. D. Gallardo, G. A. Clines, J. A. Ashley, C. J. Hilliard, J. J. Wasmuth, John Douglas Mcpherson, M. Lovett

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains ~20% of the ~5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation.

Original languageEnglish (US)
Pages (from-to)185-194
Number of pages10
JournalGenome Research
Volume5
Issue number2
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Del Mastro, R. G., Wang, L., Simmons, A. D., Gallardo, T. D., Clines, G. A., Ashley, J. A., Hilliard, C. J., Wasmuth, J. J., Mcpherson, J. D., & Lovett, M. (1995). Human chromosome-specific cDNA libraries: New tools for gene identification and genome annotation. Genome Research, 5(2), 185-194.