Human central nervous system primitive neuroectodermal tumor expressing nerve growth factor receptors: CHP707m

David L. Baker, Usha Rani Reddy, Samuel Pleasure, Mattie Hardy, Marge Williams, Margaret Tartaglione, Jaclyn A. Biegel, Beverly S. Emanuel, Patrizia Lo Presti, Barbara Kreider, John Q. Trojanowski, Audrey Evans, Amit R. Roy, Gita Venkatakrishnan, Jie Chen, Alonzo H. Ross, David E Pleasure

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A primitive neuroectodermal tumor (PNET) presented as a cerebral hemispheric mass in a 33-year-old man. Bone marrow metastases were discovered 11 months later. A cell line (CHP707m) was derived from these metastases. In culture, the cells showed features of neuronal differentiation, forming short neurites and synthesizing low-molecularweight neurofilament protein. Northern blotting showed the tumor cells express nerve growth factor (NGF) receptor messenger RNA, and fluorescence-activated cell-sorting demonstrated NGF receptors on the cell surface. Western blotting showed CHP707m NGF receptors are truncated. The receptors are functional; they bind iodine 125-labeled mouse NGF with an affinity of 1.6 × 10-9M, and short-term treatment with NGF induces expression by the tumor cells of the proto-oncogene, c-fos. Although CHP707m is the first central nervous system PNET cell line proven to express NGF receptors, immunohistological survey of tissue sections prepared from human central nervous system PNETs showed that 13 of 35 contained NGF receptor-positive tumor cells. Thus, more than one-third of such tumors might be responsive to the effects of NGF.

Original languageEnglish (US)
Pages (from-to)136-145
Number of pages10
JournalAnnals of Neurology
Volume28
Issue number2
StatePublished - Aug 1990
Externally publishedYes

Fingerprint

Nerve Growth Factor Receptors
Primitive Neuroectodermal Tumors
Central Nervous System
Nerve Growth Factor
Nerve Growth Factor Receptor
Neoplasms
Neoplasm Metastasis
fos Genes
Neurofilament Proteins
Neurites
Tumor Cell Line
Iodine
Northern Blotting
Flow Cytometry
Bone Marrow
Western Blotting
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Baker, D. L., Reddy, U. R., Pleasure, S., Hardy, M., Williams, M., Tartaglione, M., ... Pleasure, D. E. (1990). Human central nervous system primitive neuroectodermal tumor expressing nerve growth factor receptors: CHP707m. Annals of Neurology, 28(2), 136-145.

Human central nervous system primitive neuroectodermal tumor expressing nerve growth factor receptors : CHP707m. / Baker, David L.; Reddy, Usha Rani; Pleasure, Samuel; Hardy, Mattie; Williams, Marge; Tartaglione, Margaret; Biegel, Jaclyn A.; Emanuel, Beverly S.; Presti, Patrizia Lo; Kreider, Barbara; Trojanowski, John Q.; Evans, Audrey; Roy, Amit R.; Venkatakrishnan, Gita; Chen, Jie; Ross, Alonzo H.; Pleasure, David E.

In: Annals of Neurology, Vol. 28, No. 2, 08.1990, p. 136-145.

Research output: Contribution to journalArticle

Baker, DL, Reddy, UR, Pleasure, S, Hardy, M, Williams, M, Tartaglione, M, Biegel, JA, Emanuel, BS, Presti, PL, Kreider, B, Trojanowski, JQ, Evans, A, Roy, AR, Venkatakrishnan, G, Chen, J, Ross, AH & Pleasure, DE 1990, 'Human central nervous system primitive neuroectodermal tumor expressing nerve growth factor receptors: CHP707m', Annals of Neurology, vol. 28, no. 2, pp. 136-145.
Baker DL, Reddy UR, Pleasure S, Hardy M, Williams M, Tartaglione M et al. Human central nervous system primitive neuroectodermal tumor expressing nerve growth factor receptors: CHP707m. Annals of Neurology. 1990 Aug;28(2):136-145.
Baker, David L. ; Reddy, Usha Rani ; Pleasure, Samuel ; Hardy, Mattie ; Williams, Marge ; Tartaglione, Margaret ; Biegel, Jaclyn A. ; Emanuel, Beverly S. ; Presti, Patrizia Lo ; Kreider, Barbara ; Trojanowski, John Q. ; Evans, Audrey ; Roy, Amit R. ; Venkatakrishnan, Gita ; Chen, Jie ; Ross, Alonzo H. ; Pleasure, David E. / Human central nervous system primitive neuroectodermal tumor expressing nerve growth factor receptors : CHP707m. In: Annals of Neurology. 1990 ; Vol. 28, No. 2. pp. 136-145.
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abstract = "A primitive neuroectodermal tumor (PNET) presented as a cerebral hemispheric mass in a 33-year-old man. Bone marrow metastases were discovered 11 months later. A cell line (CHP707m) was derived from these metastases. In culture, the cells showed features of neuronal differentiation, forming short neurites and synthesizing low-molecularweight neurofilament protein. Northern blotting showed the tumor cells express nerve growth factor (NGF) receptor messenger RNA, and fluorescence-activated cell-sorting demonstrated NGF receptors on the cell surface. Western blotting showed CHP707m NGF receptors are truncated. The receptors are functional; they bind iodine 125-labeled mouse NGF with an affinity of 1.6 × 10-9M, and short-term treatment with NGF induces expression by the tumor cells of the proto-oncogene, c-fos. Although CHP707m is the first central nervous system PNET cell line proven to express NGF receptors, immunohistological survey of tissue sections prepared from human central nervous system PNETs showed that 13 of 35 contained NGF receptor-positive tumor cells. Thus, more than one-third of such tumors might be responsive to the effects of NGF.",
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