Abstract
Exhaled breath condensate (EBC) analysis is a developing field with tremendous promise to advance personalized, non-invasive health diagnostics as new analytical instrumentation platforms and detection methods are developed. Multiple commercially-available and researcher-built experimental samplers are reported in the literature. However, there is very limited information available to determine an effective breath sampling approach, especially regarding the dependence of breath sample metabolomic content on the collection device design and sampling methodology. This lack of an optimal standard procedure results in a range of reported results that are sometimes contradictory. Here, we present a design of a portable human EBC sampler optimized for collection and preservation of the rich metabolomic content of breath. The performance of the engineered device is compared to two commercially available breath collection devices: the RTube™ and TurboDECCS. A number of design and performance parameters are considered, including: condenser temperature stability during sampling, collection efficiency, condenser material choice, and saliva contamination in the collected breath samples. The significance of the biological content of breath samples, collected with each device, is evaluated with a set of mass spectrometry methods and was the primary factor for evaluating device performance. The design includes an adjustable mass-size threshold for aerodynamic filtering of saliva droplets from the breath flow. Engineering an inexpensive device that allows efficient collection of metalomic-rich breath samples is intended to aid further advancement in the field of breath analysis for non-invasive health diagnostic. EBC sampling from human volunteers was performed under UC Davis IRB protocol 63701-3 (09/30/2014-07/07/2017).
| Original language | English (US) |
|---|---|
| Article number | 016001 |
| Journal | Journal of Breath Research |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 2017 |
Fingerprint
Keywords
- breath analysis
- exhaled breath condensate (EBC)
- metabolomics
- non-volatile organic compounds (non-VOCs)
- portable non-invasive health diagnostic
- volatile organic compounds (VOCs)
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Human breath metabolomics using an optimized non-invasive exhaled breath condensate sampler. / Zamuruyev, Konstantin O.; Aksenov, Alexander A.; Pasamontes, Alberto; Brown, Joshua F.; Pettit, Dayna R.; Foutouhi, Soraya; Weimer, Bart C; Schivo, Michael; Kenyon, Nicholas; Delplanque, Jean Pierre; Davis, Cristina E.
In: Journal of Breath Research, Vol. 11, No. 1, 016001, 01.03.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human breath metabolomics using an optimized non-invasive exhaled breath condensate sampler
AU - Zamuruyev, Konstantin O.
AU - Aksenov, Alexander A.
AU - Pasamontes, Alberto
AU - Brown, Joshua F.
AU - Pettit, Dayna R.
AU - Foutouhi, Soraya
AU - Weimer, Bart C
AU - Schivo, Michael
AU - Kenyon, Nicholas
AU - Delplanque, Jean Pierre
AU - Davis, Cristina E
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Exhaled breath condensate (EBC) analysis is a developing field with tremendous promise to advance personalized, non-invasive health diagnostics as new analytical instrumentation platforms and detection methods are developed. Multiple commercially-available and researcher-built experimental samplers are reported in the literature. However, there is very limited information available to determine an effective breath sampling approach, especially regarding the dependence of breath sample metabolomic content on the collection device design and sampling methodology. This lack of an optimal standard procedure results in a range of reported results that are sometimes contradictory. Here, we present a design of a portable human EBC sampler optimized for collection and preservation of the rich metabolomic content of breath. The performance of the engineered device is compared to two commercially available breath collection devices: the RTube™ and TurboDECCS. A number of design and performance parameters are considered, including: condenser temperature stability during sampling, collection efficiency, condenser material choice, and saliva contamination in the collected breath samples. The significance of the biological content of breath samples, collected with each device, is evaluated with a set of mass spectrometry methods and was the primary factor for evaluating device performance. The design includes an adjustable mass-size threshold for aerodynamic filtering of saliva droplets from the breath flow. Engineering an inexpensive device that allows efficient collection of metalomic-rich breath samples is intended to aid further advancement in the field of breath analysis for non-invasive health diagnostic. EBC sampling from human volunteers was performed under UC Davis IRB protocol 63701-3 (09/30/2014-07/07/2017).
AB - Exhaled breath condensate (EBC) analysis is a developing field with tremendous promise to advance personalized, non-invasive health diagnostics as new analytical instrumentation platforms and detection methods are developed. Multiple commercially-available and researcher-built experimental samplers are reported in the literature. However, there is very limited information available to determine an effective breath sampling approach, especially regarding the dependence of breath sample metabolomic content on the collection device design and sampling methodology. This lack of an optimal standard procedure results in a range of reported results that are sometimes contradictory. Here, we present a design of a portable human EBC sampler optimized for collection and preservation of the rich metabolomic content of breath. The performance of the engineered device is compared to two commercially available breath collection devices: the RTube™ and TurboDECCS. A number of design and performance parameters are considered, including: condenser temperature stability during sampling, collection efficiency, condenser material choice, and saliva contamination in the collected breath samples. The significance of the biological content of breath samples, collected with each device, is evaluated with a set of mass spectrometry methods and was the primary factor for evaluating device performance. The design includes an adjustable mass-size threshold for aerodynamic filtering of saliva droplets from the breath flow. Engineering an inexpensive device that allows efficient collection of metalomic-rich breath samples is intended to aid further advancement in the field of breath analysis for non-invasive health diagnostic. EBC sampling from human volunteers was performed under UC Davis IRB protocol 63701-3 (09/30/2014-07/07/2017).
KW - breath analysis
KW - exhaled breath condensate (EBC)
KW - metabolomics
KW - non-volatile organic compounds (non-VOCs)
KW - portable non-invasive health diagnostic
KW - volatile organic compounds (VOCs)
UR - http://www.scopus.com/inward/record.url?scp=85015639017&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015639017&partnerID=8YFLogxK
U2 - 10.1088/1752-7163/11/1/016001
DO - 10.1088/1752-7163/11/1/016001
M3 - Article
C2 - 28004639
AN - SCOPUS:85015639017
VL - 11
JO - Journal of Breath Research
JF - Journal of Breath Research
SN - 1752-7155
IS - 1
M1 - 016001
ER -