Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA

Jie Liu, Tammy Doty, Bryan Gibson, Wolf Dietrich Heyer

Research output: Contribution to journalArticle

221 Scopus citations

Abstract

BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2 and show that it binds to ∼6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1.

Original languageEnglish (US)
Pages (from-to)1260-1262
Number of pages3
JournalNature Structural and Molecular Biology
Volume17
Issue number10
DOIs
StatePublished - Oct 2010

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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