Human brain glycogen phosphorylase: characterization of fetal cDNA and genomic sequences

Richard P. Gelinas; Byron E. Froman; Fred McElroy; Robert C. Tait; Fredric A Gorin

doi:10.1016/0169-328X(89)90052-1

Human brain glycogen phosphorylase: characterization of fetal cDNA and genomic sequences

Richard P. Gelinas, Byron E. Froman, Fred McElroy, Robert C. Tait, Fredric A Gorin

Neurology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Glycogen phosphorylase (α-1,4-d-glucan:orthophosphate d-glucosyltransferase, EC 2.4.1.1) is the rate-determining enzyme catalyzing glycogen degradation. Human brain has been demonstrated previously to express genes of both the liver and muscle isozymes of glycogen phosphorylase. In this report, a human fetal brain cDNA and genomic DNA corresponding to the brain isozyme of glycogen phosphorylase were isolated and characterized. Transcripts corresponding to this isozyme are present in human adult and fetal brain, and at low levels in other human fetal tissues. The predicted C-terminal sequence of the protein encoded by this cDNA and gene differ from that encoded by a phosphorylase cDNA isolated from a human astrocytoma cell line.

Original language	English (US)
Pages (from-to)	177-185
Number of pages	9
Journal	Molecular Brain Research
Volume	6
Issue number	2-3
DOIs	https://doi.org/10.1016/0169-328X(89)90052-1
State	Published - 1989

Keywords

Brain isozyme
Glycogen phosphorylase
Human tissue
Transcription

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(89)90052-1

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title = "Human brain glycogen phosphorylase: characterization of fetal cDNA and genomic sequences",

abstract = "Glycogen phosphorylase (α-1,4-d-glucan:orthophosphate d-glucosyltransferase, EC 2.4.1.1) is the rate-determining enzyme catalyzing glycogen degradation. Human brain has been demonstrated previously to express genes of both the liver and muscle isozymes of glycogen phosphorylase. In this report, a human fetal brain cDNA and genomic DNA corresponding to the brain isozyme of glycogen phosphorylase were isolated and characterized. Transcripts corresponding to this isozyme are present in human adult and fetal brain, and at low levels in other human fetal tissues. The predicted C-terminal sequence of the protein encoded by this cDNA and gene differ from that encoded by a phosphorylase cDNA isolated from a human astrocytoma cell line.",

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T2 - characterization of fetal cDNA and genomic sequences

AU - Gelinas, Richard P.

AU - Froman, Byron E.

AU - McElroy, Fred

AU - Tait, Robert C.

AU - Gorin, Fredric A

PY - 1989

Y1 - 1989

N2 - Glycogen phosphorylase (α-1,4-d-glucan:orthophosphate d-glucosyltransferase, EC 2.4.1.1) is the rate-determining enzyme catalyzing glycogen degradation. Human brain has been demonstrated previously to express genes of both the liver and muscle isozymes of glycogen phosphorylase. In this report, a human fetal brain cDNA and genomic DNA corresponding to the brain isozyme of glycogen phosphorylase were isolated and characterized. Transcripts corresponding to this isozyme are present in human adult and fetal brain, and at low levels in other human fetal tissues. The predicted C-terminal sequence of the protein encoded by this cDNA and gene differ from that encoded by a phosphorylase cDNA isolated from a human astrocytoma cell line.

AB - Glycogen phosphorylase (α-1,4-d-glucan:orthophosphate d-glucosyltransferase, EC 2.4.1.1) is the rate-determining enzyme catalyzing glycogen degradation. Human brain has been demonstrated previously to express genes of both the liver and muscle isozymes of glycogen phosphorylase. In this report, a human fetal brain cDNA and genomic DNA corresponding to the brain isozyme of glycogen phosphorylase were isolated and characterized. Transcripts corresponding to this isozyme are present in human adult and fetal brain, and at low levels in other human fetal tissues. The predicted C-terminal sequence of the protein encoded by this cDNA and gene differ from that encoded by a phosphorylase cDNA isolated from a human astrocytoma cell line.

KW - Brain isozyme

KW - Glycogen phosphorylase

KW - Human tissue

KW - Transcription

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Human brain glycogen phosphorylase: characterization of fetal cDNA and genomic sequences

Abstract

Keywords

ASJC Scopus subject areas

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