Human brain glycogen phosphorylase: characterization of fetal cDNA and genomic sequences

Richard P. Gelinas, Byron E. Froman, Fred McElroy, Robert C. Tait, Fredric A Gorin

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Glycogen phosphorylase (α-1,4-d-glucan:orthophosphate d-glucosyltransferase, EC 2.4.1.1) is the rate-determining enzyme catalyzing glycogen degradation. Human brain has been demonstrated previously to express genes of both the liver and muscle isozymes of glycogen phosphorylase. In this report, a human fetal brain cDNA and genomic DNA corresponding to the brain isozyme of glycogen phosphorylase were isolated and characterized. Transcripts corresponding to this isozyme are present in human adult and fetal brain, and at low levels in other human fetal tissues. The predicted C-terminal sequence of the protein encoded by this cDNA and gene differ from that encoded by a phosphorylase cDNA isolated from a human astrocytoma cell line.

Original languageEnglish (US)
Pages (from-to)177-185
Number of pages9
JournalMolecular Brain Research
Volume6
Issue number2-3
DOIs
StatePublished - 1989

Keywords

  • Brain isozyme
  • Glycogen phosphorylase
  • Human tissue
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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