Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene

Evidence for functional conservation

Roland Kanaar, Christine Troelstra, Sigrid M A Swagemakers, Jeroen Essers, Bep Smit, Jan Huib Franssen, Albert Pastink, Olga Y. Bezzubova, Jean Marie Buerstedde, Beate Clever, Wolf Dietrich Heyer, Jan H J Hoeijmakers

Research output: Contribution to journalArticle

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Abstract

Background: Homologous recombination is of eminent importance both in germ cells, to generate genetic diversity during meiosis, and in somatic cells, to safeguard DNA from genotoxic damage. The genetically well-defined RAD52 pathway is required for these processes in the yeast Saccharomyces cerevisiae. Genes similar to those in the RAD52 group have been identified in mammals. It is not known whether this conservation of primary sequence extends to conservation of function.Results: Here we report the isolation of cDNAs encoding a human and a mouse homolog of RAD54. The human (hHR54) and mouse (mHR54) proteins were 48 % identical to Rad54 and belonged to the SNF2/SW12 family, which is characterized by amino-acid motifs found in DNA- dependent ATPases. The hHR54 gene was mapped to chromosome 1p32, and the hHR54 protein was located in the nucleus. We found that the levels of hHR54 mRNA increased in late G1 phase, as has been found for RAD54 mRNA. The level of mHR54 mRNA was elevated in organs of germ cell and lymphoid development and increased mHR54 expression correlated with the meiotic phase of spermatogenesis. The hHR54 cDNA could partially complement the methyl methanesulfonate-sensitive phenotype of S. cerevisiae rad54Δ cells. Conclusions: The tissue-specific expression of mHR54 is consistent with a role for the gene in recombination. The complementation experiments show that the DNA repair function of Rad54 is conserved from yeast to humans. Our findings underscore the fundamental importance of DNA repair pathways: even though they are complex and involve multiple proteins, they seem to be functionally conserved throughout the eukaryotic kingdom.

Original languageEnglish (US)
Pages (from-to)828-838
Number of pages11
JournalCurrent Biology
Volume6
Issue number7
DOIs
StatePublished - Jul 1 1996
Externally publishedYes

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DNA repair
DNA Repair
Yeast
Saccharomyces cerevisiae
Conservation
Repair
Genes
Germ Cells
Messenger RNA
germ cells
DNA
mice
Complementary DNA
Yeasts
methyl methanesulfonate
amino acid motifs
yeasts
Methyl Methanesulfonate
Amino Acid Motifs
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Kanaar, R., Troelstra, C., Swagemakers, S. M. A., Essers, J., Smit, B., Franssen, J. H., ... Hoeijmakers, J. H. J. (1996). Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene: Evidence for functional conservation. Current Biology, 6(7), 828-838. https://doi.org/10.1016/S0960-9822(02)00606-1

Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene : Evidence for functional conservation. / Kanaar, Roland; Troelstra, Christine; Swagemakers, Sigrid M A; Essers, Jeroen; Smit, Bep; Franssen, Jan Huib; Pastink, Albert; Bezzubova, Olga Y.; Buerstedde, Jean Marie; Clever, Beate; Heyer, Wolf Dietrich; Hoeijmakers, Jan H J.

In: Current Biology, Vol. 6, No. 7, 01.07.1996, p. 828-838.

Research output: Contribution to journalArticle

Kanaar, R, Troelstra, C, Swagemakers, SMA, Essers, J, Smit, B, Franssen, JH, Pastink, A, Bezzubova, OY, Buerstedde, JM, Clever, B, Heyer, WD & Hoeijmakers, JHJ 1996, 'Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene: Evidence for functional conservation', Current Biology, vol. 6, no. 7, pp. 828-838. https://doi.org/10.1016/S0960-9822(02)00606-1
Kanaar, Roland ; Troelstra, Christine ; Swagemakers, Sigrid M A ; Essers, Jeroen ; Smit, Bep ; Franssen, Jan Huib ; Pastink, Albert ; Bezzubova, Olga Y. ; Buerstedde, Jean Marie ; Clever, Beate ; Heyer, Wolf Dietrich ; Hoeijmakers, Jan H J. / Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene : Evidence for functional conservation. In: Current Biology. 1996 ; Vol. 6, No. 7. pp. 828-838.
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AU - Essers, Jeroen

AU - Smit, Bep

AU - Franssen, Jan Huib

AU - Pastink, Albert

AU - Bezzubova, Olga Y.

AU - Buerstedde, Jean Marie

AU - Clever, Beate

AU - Heyer, Wolf Dietrich

AU - Hoeijmakers, Jan H J

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N2 - Background: Homologous recombination is of eminent importance both in germ cells, to generate genetic diversity during meiosis, and in somatic cells, to safeguard DNA from genotoxic damage. The genetically well-defined RAD52 pathway is required for these processes in the yeast Saccharomyces cerevisiae. Genes similar to those in the RAD52 group have been identified in mammals. It is not known whether this conservation of primary sequence extends to conservation of function.Results: Here we report the isolation of cDNAs encoding a human and a mouse homolog of RAD54. The human (hHR54) and mouse (mHR54) proteins were 48 % identical to Rad54 and belonged to the SNF2/SW12 family, which is characterized by amino-acid motifs found in DNA- dependent ATPases. The hHR54 gene was mapped to chromosome 1p32, and the hHR54 protein was located in the nucleus. We found that the levels of hHR54 mRNA increased in late G1 phase, as has been found for RAD54 mRNA. The level of mHR54 mRNA was elevated in organs of germ cell and lymphoid development and increased mHR54 expression correlated with the meiotic phase of spermatogenesis. The hHR54 cDNA could partially complement the methyl methanesulfonate-sensitive phenotype of S. cerevisiae rad54Δ cells. Conclusions: The tissue-specific expression of mHR54 is consistent with a role for the gene in recombination. The complementation experiments show that the DNA repair function of Rad54 is conserved from yeast to humans. Our findings underscore the fundamental importance of DNA repair pathways: even though they are complex and involve multiple proteins, they seem to be functionally conserved throughout the eukaryotic kingdom.

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