Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro

I. Lubetzki-Korn, M. Hirayama, D. H. Silberberg, A. D. Schreiber, P. A. Eccleston, David E Pleasure, T. Brenner, O. Abramsky

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Polyclonal rabbit antiserum to galactocerebroside (anti-GalC) produces titer-dependent lysis of cultured Percoll-isolated bovine and rat oligodendrocytes. In this study anti-GalC produced complement-dependent lysis of 76% of the bovine cells and 65% of the rat cells maintained for 3 to 6 days in vitro. With the concomitant addition of human umbilical cord serum fractions containing fetal alpha-fetoprotein (AFP), lysis was decreased to 31% and 39%, respectively. Control antisera (anti-complete Freund's adjuvant/albumin) showed a cytotoxicity index of 15% for bovine cells and 23% for rat cells. Neither albumin, nor normal human serum, nor any of several pregnancy-associated hormones reduced the lysis produced by anti-GalC. AFP-rich fraction reduced oligodendrocyte lysis when mixed with anti-GalC or complement, but not when first incubated with the cells. Similar findings were obtained when AFP was assayed in specific oligodendrocyte radioimmunoassays utilizing anti-GalC antibody. Our experiments indicate that AFP activity may result from its binding to anti-GalC antibody; it is possible that the Fc portion of the antibody is involved. These data provide in vitro evidence of a possible immunosuppressive role of AFP in the central nervous system.

Original languageEnglish (US)
Pages (from-to)171-180
Number of pages10
JournalAnnals of Neurology
Volume15
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

Oligodendroglia
Immune Sera
alpha-Fetoproteins
Antibodies
Albumins
Freund's Adjuvant
Umbilical Cord
Immunosuppressive Agents
galactocerebroside
human AFP protein
In Vitro Techniques
Serum
Radioimmunoassay
Central Nervous System
Hormones
Rabbits
Pregnancy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lubetzki-Korn, I., Hirayama, M., Silberberg, D. H., Schreiber, A. D., Eccleston, P. A., Pleasure, D. E., ... Abramsky, O. (1984). Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro. Annals of Neurology, 15(2), 171-180.

Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro. / Lubetzki-Korn, I.; Hirayama, M.; Silberberg, D. H.; Schreiber, A. D.; Eccleston, P. A.; Pleasure, David E; Brenner, T.; Abramsky, O.

In: Annals of Neurology, Vol. 15, No. 2, 1984, p. 171-180.

Research output: Contribution to journalArticle

Lubetzki-Korn, I, Hirayama, M, Silberberg, DH, Schreiber, AD, Eccleston, PA, Pleasure, DE, Brenner, T & Abramsky, O 1984, 'Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro', Annals of Neurology, vol. 15, no. 2, pp. 171-180.
Lubetzki-Korn I, Hirayama M, Silberberg DH, Schreiber AD, Eccleston PA, Pleasure DE et al. Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro. Annals of Neurology. 1984;15(2):171-180.
Lubetzki-Korn, I. ; Hirayama, M. ; Silberberg, D. H. ; Schreiber, A. D. ; Eccleston, P. A. ; Pleasure, David E ; Brenner, T. ; Abramsky, O. / Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro. In: Annals of Neurology. 1984 ; Vol. 15, No. 2. pp. 171-180.
@article{d738c14b5e2146dcb96272b6a7bc2ff7,
title = "Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro",
abstract = "Polyclonal rabbit antiserum to galactocerebroside (anti-GalC) produces titer-dependent lysis of cultured Percoll-isolated bovine and rat oligodendrocytes. In this study anti-GalC produced complement-dependent lysis of 76{\%} of the bovine cells and 65{\%} of the rat cells maintained for 3 to 6 days in vitro. With the concomitant addition of human umbilical cord serum fractions containing fetal alpha-fetoprotein (AFP), lysis was decreased to 31{\%} and 39{\%}, respectively. Control antisera (anti-complete Freund's adjuvant/albumin) showed a cytotoxicity index of 15{\%} for bovine cells and 23{\%} for rat cells. Neither albumin, nor normal human serum, nor any of several pregnancy-associated hormones reduced the lysis produced by anti-GalC. AFP-rich fraction reduced oligodendrocyte lysis when mixed with anti-GalC or complement, but not when first incubated with the cells. Similar findings were obtained when AFP was assayed in specific oligodendrocyte radioimmunoassays utilizing anti-GalC antibody. Our experiments indicate that AFP activity may result from its binding to anti-GalC antibody; it is possible that the Fc portion of the antibody is involved. These data provide in vitro evidence of a possible immunosuppressive role of AFP in the central nervous system.",
author = "I. Lubetzki-Korn and M. Hirayama and Silberberg, {D. H.} and Schreiber, {A. D.} and Eccleston, {P. A.} and Pleasure, {David E} and T. Brenner and O. Abramsky",
year = "1984",
language = "English (US)",
volume = "15",
pages = "171--180",
journal = "Annals of Neurology",
issn = "0364-5134",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Human alpha-fetoprotein-rich fraction inhibits galactocerebroside antibody-mediated lysis of oligodendrocytes in vitro

AU - Lubetzki-Korn, I.

AU - Hirayama, M.

AU - Silberberg, D. H.

AU - Schreiber, A. D.

AU - Eccleston, P. A.

AU - Pleasure, David E

AU - Brenner, T.

AU - Abramsky, O.

PY - 1984

Y1 - 1984

N2 - Polyclonal rabbit antiserum to galactocerebroside (anti-GalC) produces titer-dependent lysis of cultured Percoll-isolated bovine and rat oligodendrocytes. In this study anti-GalC produced complement-dependent lysis of 76% of the bovine cells and 65% of the rat cells maintained for 3 to 6 days in vitro. With the concomitant addition of human umbilical cord serum fractions containing fetal alpha-fetoprotein (AFP), lysis was decreased to 31% and 39%, respectively. Control antisera (anti-complete Freund's adjuvant/albumin) showed a cytotoxicity index of 15% for bovine cells and 23% for rat cells. Neither albumin, nor normal human serum, nor any of several pregnancy-associated hormones reduced the lysis produced by anti-GalC. AFP-rich fraction reduced oligodendrocyte lysis when mixed with anti-GalC or complement, but not when first incubated with the cells. Similar findings were obtained when AFP was assayed in specific oligodendrocyte radioimmunoassays utilizing anti-GalC antibody. Our experiments indicate that AFP activity may result from its binding to anti-GalC antibody; it is possible that the Fc portion of the antibody is involved. These data provide in vitro evidence of a possible immunosuppressive role of AFP in the central nervous system.

AB - Polyclonal rabbit antiserum to galactocerebroside (anti-GalC) produces titer-dependent lysis of cultured Percoll-isolated bovine and rat oligodendrocytes. In this study anti-GalC produced complement-dependent lysis of 76% of the bovine cells and 65% of the rat cells maintained for 3 to 6 days in vitro. With the concomitant addition of human umbilical cord serum fractions containing fetal alpha-fetoprotein (AFP), lysis was decreased to 31% and 39%, respectively. Control antisera (anti-complete Freund's adjuvant/albumin) showed a cytotoxicity index of 15% for bovine cells and 23% for rat cells. Neither albumin, nor normal human serum, nor any of several pregnancy-associated hormones reduced the lysis produced by anti-GalC. AFP-rich fraction reduced oligodendrocyte lysis when mixed with anti-GalC or complement, but not when first incubated with the cells. Similar findings were obtained when AFP was assayed in specific oligodendrocyte radioimmunoassays utilizing anti-GalC antibody. Our experiments indicate that AFP activity may result from its binding to anti-GalC antibody; it is possible that the Fc portion of the antibody is involved. These data provide in vitro evidence of a possible immunosuppressive role of AFP in the central nervous system.

UR - http://www.scopus.com/inward/record.url?scp=0021367723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021367723&partnerID=8YFLogxK

M3 - Article

C2 - 6200057

AN - SCOPUS:0021367723

VL - 15

SP - 171

EP - 180

JO - Annals of Neurology

JF - Annals of Neurology

SN - 0364-5134

IS - 2

ER -