Abstract
The effect of human interferons (IFNs) (alpha, beta, and gamma) on the in vitro replication of AIDS viruses (LAV, HTLV-III, and ARV-2) in human peripheral blood lymphocytes was investigated. At the time of peak virus production, IFN-α preparations (leukocyte, Namalwa, α1, and α2) at 100 U/ml, suppressed LAV, HTLV-III, and ARV-2 replication as measured by reverse transcriptase (RT) activity by greater than 50%. This suppression was dose dependent and high dosages (500 U/ml) of IFN-α resulted in almost complete suppression of RT activities (77–99%). A low dose (100 U/ml) of IFN-β suppressed all three AIDS viruses by 75%. In contrast, human IFN-α at a dose range from 100 U/ml to 500 U/ml had no significant effect on the production of infectious viruses. These results indicate that only IFN-α and -β are effective against LAV, HTLV-III, and ARV-2 replication. A continuous supply of IFN appeared to be essential for the constant suppression of RT activity. In fact, upon termination of single IFN treatment, enhanced virus production resulted.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 143-152 |
| Number of pages | 10 |
| Journal | Journal of Interferon Research |
| Volume | 6 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1986 |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Human Alpha- and Beta-Interferon But Not Gamma- Suppress the In Vitro Replication of LAV, HTLV-III, and ARV-2. / Yamamoto, Janet K.; Barré Sinoussi, Françoise; Bolton, Veronica; Pedersen, Niels C; Gardner, Murray B.
In: Journal of Interferon Research, Vol. 6, No. 2, 1986, p. 143-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human Alpha- and Beta-Interferon But Not Gamma- Suppress the In Vitro Replication of LAV, HTLV-III, and ARV-2
AU - Yamamoto, Janet K.
AU - Barré Sinoussi, Françoise
AU - Bolton, Veronica
AU - Pedersen, Niels C
AU - Gardner, Murray B.
PY - 1986
Y1 - 1986
N2 - The effect of human interferons (IFNs) (alpha, beta, and gamma) on the in vitro replication of AIDS viruses (LAV, HTLV-III, and ARV-2) in human peripheral blood lymphocytes was investigated. At the time of peak virus production, IFN-α preparations (leukocyte, Namalwa, α1, and α2) at 100 U/ml, suppressed LAV, HTLV-III, and ARV-2 replication as measured by reverse transcriptase (RT) activity by greater than 50%. This suppression was dose dependent and high dosages (500 U/ml) of IFN-α resulted in almost complete suppression of RT activities (77–99%). A low dose (100 U/ml) of IFN-β suppressed all three AIDS viruses by 75%. In contrast, human IFN-α at a dose range from 100 U/ml to 500 U/ml had no significant effect on the production of infectious viruses. These results indicate that only IFN-α and -β are effective against LAV, HTLV-III, and ARV-2 replication. A continuous supply of IFN appeared to be essential for the constant suppression of RT activity. In fact, upon termination of single IFN treatment, enhanced virus production resulted.
AB - The effect of human interferons (IFNs) (alpha, beta, and gamma) on the in vitro replication of AIDS viruses (LAV, HTLV-III, and ARV-2) in human peripheral blood lymphocytes was investigated. At the time of peak virus production, IFN-α preparations (leukocyte, Namalwa, α1, and α2) at 100 U/ml, suppressed LAV, HTLV-III, and ARV-2 replication as measured by reverse transcriptase (RT) activity by greater than 50%. This suppression was dose dependent and high dosages (500 U/ml) of IFN-α resulted in almost complete suppression of RT activities (77–99%). A low dose (100 U/ml) of IFN-β suppressed all three AIDS viruses by 75%. In contrast, human IFN-α at a dose range from 100 U/ml to 500 U/ml had no significant effect on the production of infectious viruses. These results indicate that only IFN-α and -β are effective against LAV, HTLV-III, and ARV-2 replication. A continuous supply of IFN appeared to be essential for the constant suppression of RT activity. In fact, upon termination of single IFN treatment, enhanced virus production resulted.
UR - http://www.scopus.com/inward/record.url?scp=0022573263&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022573263&partnerID=8YFLogxK
U2 - 10.1089/jir.1986.6.143
DO - 10.1089/jir.1986.6.143
M3 - Article
C2 - 2425014
AN - SCOPUS:0022573263
VL - 6
SP - 143
EP - 152
JO - Journal of Interferon Research
JF - Journal of Interferon Research
SN - 0197-8357
IS - 2
ER -