Human Alpha- and Beta-Interferon But Not Gamma- Suppress the In Vitro Replication of LAV, HTLV-III, and ARV-2

Janet K. Yamamoto, Françoise Barré Sinoussi, Veronica Bolton, Niels C Pedersen, Murray B. Gardner

Research output: Contribution to journalArticle

126 Scopus citations

Abstract

The effect of human interferons (IFNs) (alpha, beta, and gamma) on the in vitro replication of AIDS viruses (LAV, HTLV-III, and ARV-2) in human peripheral blood lymphocytes was investigated. At the time of peak virus production, IFN-α preparations (leukocyte, Namalwa, α1, and α2) at 100 U/ml, suppressed LAV, HTLV-III, and ARV-2 replication as measured by reverse transcriptase (RT) activity by greater than 50%. This suppression was dose dependent and high dosages (500 U/ml) of IFN-α resulted in almost complete suppression of RT activities (77–99%). A low dose (100 U/ml) of IFN-β suppressed all three AIDS viruses by 75%. In contrast, human IFN-α at a dose range from 100 U/ml to 500 U/ml had no significant effect on the production of infectious viruses. These results indicate that only IFN-α and -β are effective against LAV, HTLV-III, and ARV-2 replication. A continuous supply of IFN appeared to be essential for the constant suppression of RT activity. In fact, upon termination of single IFN treatment, enhanced virus production resulted.

Original languageEnglish (US)
Pages (from-to)143-152
Number of pages10
JournalJournal of Interferon Research
Volume6
Issue number2
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Immunology
  • Virology

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