Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family

Scott M. Hammond, Yelena M. Altshuller, Tsung Chang Sung, Simon A. Rudge, Kristine Rose, JoAnne Engebrecht, Andrew J. Morris, Michael A. Frohman

Research output: Contribution to journalArticle

572 Citations (Scopus)

Abstract

Activation of phosphatidylcholine-specific phospholipase D (PLD) has been implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. We report here the identification of the first human PLD cDNA, which defines a new and highly conserved gene family. Characterization of recombinant human PLD1 reveals that it is membrane-associated, selective for phosphatidylcholine, stimulated by phosphatidylinositol 4,5-bisphosphate, activated by the monomeric G-protein ADP-ribosylation factor-1, and inhibited by oleate. PLD1 likely encodes the gene product responsible for the most widely studied endogenous PLD activity.

Original languageEnglish (US)
Pages (from-to)29640-29643
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number50
DOIs
StatePublished - Dec 15 1995
Externally publishedYes

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ADP-Ribosylation Factors
Phospholipase D
Phosphatidylcholines
Genes
ADP-Ribosylation Factor 1
Membranes
Signal transduction
Forensic Anthropology
Monomeric GTP-Binding Proteins
Oleic Acid
Phosphatidylinositols
Mitosis
Signal Transduction
Complementary DNA
Chemical activation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hammond, S. M., Altshuller, Y. M., Sung, T. C., Rudge, S. A., Rose, K., Engebrecht, J., ... Frohman, M. A. (1995). Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family. Journal of Biological Chemistry, 270(50), 29640-29643. https://doi.org/10.1074/jbc.270.50.29640

Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family. / Hammond, Scott M.; Altshuller, Yelena M.; Sung, Tsung Chang; Rudge, Simon A.; Rose, Kristine; Engebrecht, JoAnne; Morris, Andrew J.; Frohman, Michael A.

In: Journal of Biological Chemistry, Vol. 270, No. 50, 15.12.1995, p. 29640-29643.

Research output: Contribution to journalArticle

Hammond, SM, Altshuller, YM, Sung, TC, Rudge, SA, Rose, K, Engebrecht, J, Morris, AJ & Frohman, MA 1995, 'Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family', Journal of Biological Chemistry, vol. 270, no. 50, pp. 29640-29643. https://doi.org/10.1074/jbc.270.50.29640
Hammond, Scott M. ; Altshuller, Yelena M. ; Sung, Tsung Chang ; Rudge, Simon A. ; Rose, Kristine ; Engebrecht, JoAnne ; Morris, Andrew J. ; Frohman, Michael A. / Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 50. pp. 29640-29643.
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