Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family

Scott M. Hammond; Yelena M. Altshuller; Tsung Chang Sung; Simon A. Rudge; Kristine Rose; JoAnne Engebrecht; Andrew J. Morris; Michael A. Frohman

doi:10.1074/jbc.270.50.29640

Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family

Scott M. Hammond, Yelena M. Altshuller, Tsung Chang Sung, Simon A. Rudge, Kristine Rose, JoAnne Engebrecht, Andrew J. Morris, Michael A. Frohman

Research output: Contribution to journal › Article

573 Scopus citations

Abstract

Activation of phosphatidylcholine-specific phospholipase D (PLD) has been implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. We report here the identification of the first human PLD cDNA, which defines a new and highly conserved gene family. Characterization of recombinant human PLD1 reveals that it is membrane-associated, selective for phosphatidylcholine, stimulated by phosphatidylinositol 4,5-bisphosphate, activated by the monomeric G-protein ADP-ribosylation factor-1, and inhibited by oleate. PLD1 likely encodes the gene product responsible for the most widely studied endogenous PLD activity.

Original language	English (US)
Pages (from-to)	29640-29643
Number of pages	4
Journal	Journal of Biological Chemistry
Volume	270
Issue number	50
DOIs	https://doi.org/10.1074/jbc.270.50.29640
State	Published - Dec 15 1995
Externally published	Yes

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ASJC Scopus subject areas

Biochemistry

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Hammond, S. M., Altshuller, Y. M., Sung, T. C., Rudge, S. A., Rose, K., Engebrecht, J., Morris, A. J., & Frohman, M. A. (1995). Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family. Journal of Biological Chemistry, 270(50), 29640-29643. https://doi.org/10.1074/jbc.270.50.29640

Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family. / Hammond, Scott M.; Altshuller, Yelena M.; Sung, Tsung Chang; Rudge, Simon A.; Rose, Kristine; Engebrecht, JoAnne; Morris, Andrew J.; Frohman, Michael A.

In: Journal of Biological Chemistry, Vol. 270, No. 50, 15.12.1995, p. 29640-29643.

Research output: Contribution to journal › Article

Hammond, Scott M. ; Altshuller, Yelena M. ; Sung, Tsung Chang ; Rudge, Simon A. ; Rose, Kristine ; Engebrecht, JoAnne ; Morris, Andrew J. ; Frohman, Michael A. / Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 50. pp. 29640-29643.

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