How to make calcium-sensitive minielectrodes

Roger C. Thomas, Donald M Bers

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


In this protocol we describe how to make and use minielectrodes for measuring [Ca2+] in small volumes of solution. The minielectrodes are 2 mm in diameter and have sufficiently low resistances to be used with a standard pH meter. They are made by dipping polyethylene or borosilicate glass tubes (~5 cm long) in a membrane solution. Although the chemicals used to make these Ca2+-sensitive minielectrodes are expensive, they can be used to make hundreds of electrodes, each with a useful life of several months.

Original languageEnglish (US)
Pages (from-to)370-373
Number of pages4
JournalCold Spring Harbor Protocols
Issue number4
StatePublished - 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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