How to make calcium-sensitive minielectrodes

Roger C. Thomas, Donald M Bers

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this protocol we describe how to make and use minielectrodes for measuring [Ca2+] in small volumes of solution. The minielectrodes are 2 mm in diameter and have sufficiently low resistances to be used with a standard pH meter. They are made by dipping polyethylene or borosilicate glass tubes (~5 cm long) in a membrane solution. Although the chemicals used to make these Ca2+-sensitive minielectrodes are expensive, they can be used to make hundreds of electrodes, each with a useful life of several months.

Original languageEnglish (US)
Pages (from-to)370-373
Number of pages4
JournalCold Spring Harbor Protocols
Volume8
Issue number4
DOIs
StatePublished - 2013

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pH meters
Calcium
Borosilicate glass
Polyethylene
Glass
Electrodes
Membranes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

How to make calcium-sensitive minielectrodes. / Thomas, Roger C.; Bers, Donald M.

In: Cold Spring Harbor Protocols, Vol. 8, No. 4, 2013, p. 370-373.

Research output: Contribution to journalArticle

Thomas, Roger C. ; Bers, Donald M. / How to make calcium-sensitive minielectrodes. In: Cold Spring Harbor Protocols. 2013 ; Vol. 8, No. 4. pp. 370-373.
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