How to make calcium-sensitive microelectrodes

Roger C. Thomas; Donald M Bers

doi:10.1101/pdb.prot072868

How to make calcium-sensitive microelectrodes

Roger C. Thomas, Donald M Bers

Pharmacology

Research output: Contribution to journal › Article

2 Scopus citations

Abstract

Ca²⁺-sensitive microelectrodes (CaSMs) directly measure the pCa at their tip, which can be in a small extracellular space or inside a large and robust cell. They do not add to buffering and do not require expensive equipment. But they are time-consuming to make, require a reference electrode in the same location, and tend to create a leak around the point of insertion. In addition, CaSMs only work well with a tip diameter of >1 μm. In this protocol, we describe how to make and use the electrodes and briefly consider possible problems.

Original language	English (US)
Pages (from-to)	374-378
Number of pages	5
Journal	Cold Spring Harbor Protocols
Volume	8
Issue number	4
DOIs	https://doi.org/10.1101/pdb.prot072868
State	Published - 2013

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1101/pdb.prot072868

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Thomas, R. C., & Bers, D. M. (2013). How to make calcium-sensitive microelectrodes. Cold Spring Harbor Protocols, 8(4), 374-378. https://doi.org/10.1101/pdb.prot072868

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