Abstract
Ca2+-sensitive microelectrodes (CaSMs) directly measure the pCa at their tip, which can be in a small extracellular space or inside a large and robust cell. They do not add to buffering and do not require expensive equipment. But they are time-consuming to make, require a reference electrode in the same location, and tend to create a leak around the point of insertion. In addition, CaSMs only work well with a tip diameter of >1 μm. In this protocol, we describe how to make and use the electrodes and briefly consider possible problems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 374-378 |
| Number of pages | 5 |
| Journal | Cold Spring Harbor Protocols |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2013 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
How to make calcium-sensitive microelectrodes. / Thomas, Roger C.; Bers, Donald M.
In: Cold Spring Harbor Protocols, Vol. 8, No. 4, 2013, p. 374-378.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - How to make calcium-sensitive microelectrodes
AU - Thomas, Roger C.
AU - Bers, Donald M
PY - 2013
Y1 - 2013
N2 - Ca2+-sensitive microelectrodes (CaSMs) directly measure the pCa at their tip, which can be in a small extracellular space or inside a large and robust cell. They do not add to buffering and do not require expensive equipment. But they are time-consuming to make, require a reference electrode in the same location, and tend to create a leak around the point of insertion. In addition, CaSMs only work well with a tip diameter of >1 μm. In this protocol, we describe how to make and use the electrodes and briefly consider possible problems.
AB - Ca2+-sensitive microelectrodes (CaSMs) directly measure the pCa at their tip, which can be in a small extracellular space or inside a large and robust cell. They do not add to buffering and do not require expensive equipment. But they are time-consuming to make, require a reference electrode in the same location, and tend to create a leak around the point of insertion. In addition, CaSMs only work well with a tip diameter of >1 μm. In this protocol, we describe how to make and use the electrodes and briefly consider possible problems.
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UR - http://www.scopus.com/inward/citedby.url?scp=84875878061&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot072868
DO - 10.1101/pdb.prot072868
M3 - Article
C2 - 23547154
AN - SCOPUS:84875878061
VL - 8
SP - 374
EP - 378
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1559-6095
IS - 4
ER -