How nematode sperm crawl

Dean Bottino, Alexander Mogilner, Tom Roberts, Murray Stewart, George Oster

Research output: Contribution to journalArticle

162 Scopus citations

Abstract

Sperm of the nematode, Ascaris suum, crawl using lamellipodial protrusion, adhesion and retraction, a process analogous to the amoeboid motility of other eukaryotic cells. However, rather than employing an actin cytoskeleton to generate locomotion, nematode sperm use the major sperm protein (MSP). Moreover, nematode sperm lack detectable molecular motors or the battery of actin-binding proteins that characterize actin-based motility. The Ascaris system provides a simple 'stripped down' version of a crawling cell in which to examine the basic mechanism of cell locomotion independently of other cellular functions that involve the cytoskeleton. Here we present a mechanochemical analysis of crawling in Ascaris sperm. We construct a finite element model wherein (a) localized filament polymerization and bundling generate the force for lamellipodial extension and (b) energy stored in the gel formed from the filament bundles at the leading edge is subsequently used to produce the contraction that pulls the rear of the cell forward. The model reproduces the major features of crawling sperm and provides a framework in which amoeboid cell motility can be analyzed. Although the model refers primarily to the locomotion of nematode sperm, it has important implications for the mechanics of actin-based cell motility.

Original languageEnglish (US)
Pages (from-to)367-384
Number of pages18
JournalJournal of Cell Science
Volume115
Issue number2
StatePublished - Jan 15 2002
Externally publishedYes

Keywords

  • Amoeboid movements
  • Cell motility
  • Cytoskeleton
  • Major sperm protein
  • Nematode sperm cell

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Bottino, D., Mogilner, A., Roberts, T., Stewart, M., & Oster, G. (2002). How nematode sperm crawl. Journal of Cell Science, 115(2), 367-384.