Host marrow stem cell potential and engraftability at varying times after low-dose whole-body irradiation

F. Marc Stewart, Suju Zhong, Jean François Lambert, Gerald A. Colvin, Mehrdad Abedi, Mark S. Dooner, Christina I. McAuliffe, Man Wang, Chung Hsieh, Peter J. Quesenberry

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

High levels of chimerism in syngeneic BALB/c transplants were reported when hosts were exposed to 1 Gy (100 cGy) whole body irradiation (WBI) and infused with 40 × 10 6 marrow cells. The recovery of host stem cells and alterations of enhanced host engraftability at varying times after 1 Gy WBI have now been evaluated in this study. Male BALB/c marrow (40 × 10 6 cells) was infused into female BALB/c hosts immediately or at 6, 12, and 24 weeks after 1 Gy WBI of host female BALB/c mice; engraftment percentages 8 weeks after cell injection at week 0, 6, 12, or 24 were 68% ± 12%, 45% ± 15%, 51% ± 12%, or 20% ± 8%, respectively. Eight-week engraftment levels in nonirradiated hosts average 7.7%. Conversely, engraftable stem cells measured at 8 weeks postengraftment in 1 Gyexposed hosts were reduced to 8.6% ± 3% of nonirradiated mice at time 0, 35% ± 12% 6 weeks later, 49% ± 10% at 3 months, and 21% ± 7% at 6 months. Engraftment was still increased and stem cell decreased 1 year after 1 Gy. Furthermore, the primary cells transplanted into 1 Gy hosts can be serially transplanted, and the predominant effect of 1 Gy is directly on engrafting stem cells and not through accessory cells. These data show that transplantation in 1 Gy mice may be delayed until recovery of hematopoiesis, suggesting strategies in allogeneic transplantation to avoid the adverse effects of cytokine storm. The incomplete recovery of engraftable stem cells out to 12 months indicates that stem cell expansion, especially in patients previously treated with radiomimetic drugs, may not be feasible.

Original languageEnglish (US)
Pages (from-to)1246-1251
Number of pages6
JournalBlood
Volume98
Issue number4
DOIs
StatePublished - Aug 15 2001
Externally publishedYes

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Whole-Body Irradiation
Stem cells
Dosimetry
Stem Cells
Bone Marrow
Irradiation
Recovery
Transplants
Chimerism
Homologous Transplantation
Hematopoiesis
Accessories
Transplantation
Cells
Cytokines
Injections
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Hematology

Cite this

Marc Stewart, F., Zhong, S., Lambert, J. F., Colvin, G. A., Abedi, M., Dooner, M. S., ... Quesenberry, P. J. (2001). Host marrow stem cell potential and engraftability at varying times after low-dose whole-body irradiation. Blood, 98(4), 1246-1251. https://doi.org/10.1182/blood.V98.4.1246

Host marrow stem cell potential and engraftability at varying times after low-dose whole-body irradiation. / Marc Stewart, F.; Zhong, Suju; Lambert, Jean François; Colvin, Gerald A.; Abedi, Mehrdad; Dooner, Mark S.; McAuliffe, Christina I.; Wang, Man; Hsieh, Chung; Quesenberry, Peter J.

In: Blood, Vol. 98, No. 4, 15.08.2001, p. 1246-1251.

Research output: Contribution to journalArticle

Marc Stewart, F, Zhong, S, Lambert, JF, Colvin, GA, Abedi, M, Dooner, MS, McAuliffe, CI, Wang, M, Hsieh, C & Quesenberry, PJ 2001, 'Host marrow stem cell potential and engraftability at varying times after low-dose whole-body irradiation', Blood, vol. 98, no. 4, pp. 1246-1251. https://doi.org/10.1182/blood.V98.4.1246
Marc Stewart, F. ; Zhong, Suju ; Lambert, Jean François ; Colvin, Gerald A. ; Abedi, Mehrdad ; Dooner, Mark S. ; McAuliffe, Christina I. ; Wang, Man ; Hsieh, Chung ; Quesenberry, Peter J. / Host marrow stem cell potential and engraftability at varying times after low-dose whole-body irradiation. In: Blood. 2001 ; Vol. 98, No. 4. pp. 1246-1251.
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title = "Host marrow stem cell potential and engraftability at varying times after low-dose whole-body irradiation",
abstract = "High levels of chimerism in syngeneic BALB/c transplants were reported when hosts were exposed to 1 Gy (100 cGy) whole body irradiation (WBI) and infused with 40 × 10 6 marrow cells. The recovery of host stem cells and alterations of enhanced host engraftability at varying times after 1 Gy WBI have now been evaluated in this study. Male BALB/c marrow (40 × 10 6 cells) was infused into female BALB/c hosts immediately or at 6, 12, and 24 weeks after 1 Gy WBI of host female BALB/c mice; engraftment percentages 8 weeks after cell injection at week 0, 6, 12, or 24 were 68{\%} ± 12{\%}, 45{\%} ± 15{\%}, 51{\%} ± 12{\%}, or 20{\%} ± 8{\%}, respectively. Eight-week engraftment levels in nonirradiated hosts average 7.7{\%}. Conversely, engraftable stem cells measured at 8 weeks postengraftment in 1 Gyexposed hosts were reduced to 8.6{\%} ± 3{\%} of nonirradiated mice at time 0, 35{\%} ± 12{\%} 6 weeks later, 49{\%} ± 10{\%} at 3 months, and 21{\%} ± 7{\%} at 6 months. Engraftment was still increased and stem cell decreased 1 year after 1 Gy. Furthermore, the primary cells transplanted into 1 Gy hosts can be serially transplanted, and the predominant effect of 1 Gy is directly on engrafting stem cells and not through accessory cells. These data show that transplantation in 1 Gy mice may be delayed until recovery of hematopoiesis, suggesting strategies in allogeneic transplantation to avoid the adverse effects of cytokine storm. The incomplete recovery of engraftable stem cells out to 12 months indicates that stem cell expansion, especially in patients previously treated with radiomimetic drugs, may not be feasible.",
author = "{Marc Stewart}, F. and Suju Zhong and Lambert, {Jean Fran{\cc}ois} and Colvin, {Gerald A.} and Mehrdad Abedi and Dooner, {Mark S.} and McAuliffe, {Christina I.} and Man Wang and Chung Hsieh and Quesenberry, {Peter J.}",
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AU - Lambert, Jean François

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AU - Abedi, Mehrdad

AU - Dooner, Mark S.

AU - McAuliffe, Christina I.

AU - Wang, Man

AU - Hsieh, Chung

AU - Quesenberry, Peter J.

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N2 - High levels of chimerism in syngeneic BALB/c transplants were reported when hosts were exposed to 1 Gy (100 cGy) whole body irradiation (WBI) and infused with 40 × 10 6 marrow cells. The recovery of host stem cells and alterations of enhanced host engraftability at varying times after 1 Gy WBI have now been evaluated in this study. Male BALB/c marrow (40 × 10 6 cells) was infused into female BALB/c hosts immediately or at 6, 12, and 24 weeks after 1 Gy WBI of host female BALB/c mice; engraftment percentages 8 weeks after cell injection at week 0, 6, 12, or 24 were 68% ± 12%, 45% ± 15%, 51% ± 12%, or 20% ± 8%, respectively. Eight-week engraftment levels in nonirradiated hosts average 7.7%. Conversely, engraftable stem cells measured at 8 weeks postengraftment in 1 Gyexposed hosts were reduced to 8.6% ± 3% of nonirradiated mice at time 0, 35% ± 12% 6 weeks later, 49% ± 10% at 3 months, and 21% ± 7% at 6 months. Engraftment was still increased and stem cell decreased 1 year after 1 Gy. Furthermore, the primary cells transplanted into 1 Gy hosts can be serially transplanted, and the predominant effect of 1 Gy is directly on engrafting stem cells and not through accessory cells. These data show that transplantation in 1 Gy mice may be delayed until recovery of hematopoiesis, suggesting strategies in allogeneic transplantation to avoid the adverse effects of cytokine storm. The incomplete recovery of engraftable stem cells out to 12 months indicates that stem cell expansion, especially in patients previously treated with radiomimetic drugs, may not be feasible.

AB - High levels of chimerism in syngeneic BALB/c transplants were reported when hosts were exposed to 1 Gy (100 cGy) whole body irradiation (WBI) and infused with 40 × 10 6 marrow cells. The recovery of host stem cells and alterations of enhanced host engraftability at varying times after 1 Gy WBI have now been evaluated in this study. Male BALB/c marrow (40 × 10 6 cells) was infused into female BALB/c hosts immediately or at 6, 12, and 24 weeks after 1 Gy WBI of host female BALB/c mice; engraftment percentages 8 weeks after cell injection at week 0, 6, 12, or 24 were 68% ± 12%, 45% ± 15%, 51% ± 12%, or 20% ± 8%, respectively. Eight-week engraftment levels in nonirradiated hosts average 7.7%. Conversely, engraftable stem cells measured at 8 weeks postengraftment in 1 Gyexposed hosts were reduced to 8.6% ± 3% of nonirradiated mice at time 0, 35% ± 12% 6 weeks later, 49% ± 10% at 3 months, and 21% ± 7% at 6 months. Engraftment was still increased and stem cell decreased 1 year after 1 Gy. Furthermore, the primary cells transplanted into 1 Gy hosts can be serially transplanted, and the predominant effect of 1 Gy is directly on engrafting stem cells and not through accessory cells. These data show that transplantation in 1 Gy mice may be delayed until recovery of hematopoiesis, suggesting strategies in allogeneic transplantation to avoid the adverse effects of cytokine storm. The incomplete recovery of engraftable stem cells out to 12 months indicates that stem cell expansion, especially in patients previously treated with radiomimetic drugs, may not be feasible.

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