Host alternation of chikungunya virus increases fitness while restricting population diversity and adaptability to novel selective pressures

Lark L Schneider, Marco Vignuzzi

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

The mechanisms by which RNA arboviruses, including chikungunya virus (CHIKV), evolve and maintain the ability to infect vertebrate and invertebrate hosts are poorly understood. To understand how host specificity shapes arbovirus populations, we studied CHIKV populations passaged alternately between invertebratze and vertebrate cells (invertebrate 7 vertebrate) to sim ulate natural alternation and contrasted the results with those for populations that were artificially released from cycling by passage in single cell types. These CHIKV populations were characterized by measuring genetic diversity, changes in fitness, and adaptability to novel selective pressures. The greatest fitness increases were observed in alternately passaged CHIKV, without drastic changes in population diversity. The greatest increases in genetic diversity were observed after serial passage and correlated with greater adaptability. These results suggest an evolutionary trade-off between maintaining fitness for invertebrate7vertebrate cell cycling, where maximum adaptability is possible only via enhanced population diversity and extensive exploration of sequence space.

Original languageEnglish (US)
Pages (from-to)1025-1035
Number of pages11
JournalJournal of Virology
Volume85
Issue number2
DOIs
StatePublished - Jan 2011
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology

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