Hormone synergism in the in vitro production of the mouse mammary tumor virus

Robert Cardiff, L. J T Young, R. L. Ashley

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The production of mouse mammary tumor virus (MMTV) in primary cell cultures of BALB/cfC3H mammary tumor cells was measured by radioimmune assay and RNA dependent DNA polymerase activity. Maximum virus production was dependent on cell density, nutritional milieu, and hormone supplementation. The addition of insulin (I), estradiol 17β (E2), progesterone (P), prolactin (PRL), or thyroxine (T3) alone had little or no effect on MMTV production. Hydrocortisone (F) had a primary stimulatory effect. The combination of I, F, and T3 increased MMTV levels. The combinations containing I, F, and E2 had the greatest stimulatory effect. The stimulation of MMTV production was dose dependent. These experiments demonstrate that a variety of hormones act in a synergistic manner to stimulate MMTV production.

Original languageEnglish (US)
Pages (from-to)117-129
Number of pages13
JournalJournal of Toxicology and Environmental Health
Volume1
Issue numbersup.1
StatePublished - Dec 1 1976

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Mouse mammary tumor virus
Hormones
Viruses
Tumors
Primary Cell Culture
Cortisol
RNA-Directed DNA Polymerase
Thyroxine
Prolactin
Insulin
Progesterone
Hydrocortisone
Estradiol
RNA
Cell culture
Cell Count
In Vitro Techniques
Assays
Breast Neoplasms
DNA

ASJC Scopus subject areas

  • Toxicology
  • Pollution

Cite this

Hormone synergism in the in vitro production of the mouse mammary tumor virus. / Cardiff, Robert; Young, L. J T; Ashley, R. L.

In: Journal of Toxicology and Environmental Health, Vol. 1, No. sup.1, 01.12.1976, p. 117-129.

Research output: Contribution to journalArticle

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