Homologous recombination between human immunodeficiency viral DNAs in cultured human cells: Analysis of the factors influencing recombination

S. Kalyanaraman, R. Jannoun-Nasr, D. York, Paul A Luciw, R. Robinson, A. Srinivasan

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Recombination between HIV DNAs was analyzed using DNA transfection in cell cultures and the optimal conditions for efficient recombination were determined. Recombinant plasmid DNA substrates were constructed from HIV proviral DNAs and the success of recombination was measured by the production of viable hybrid virus. The process of recombination between HIV DNAs was shown to be i) dependent on homology between the truncated HIV DNAs and ii) maximum with concentrations of the truncated DNAs 3μg and above. HIV isolates with heterogeneity in their primary sequence, thus offer an ideal system for the analysis of the requirement of homologous recombination. In addition, recombination methodology would be useful for generating hybrid HIVs for the analysis of specific viral gene functions.

Original languageEnglish (US)
Pages (from-to)1051-1060
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume157
Issue number3
DOIs
StatePublished - Dec 30 1988

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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