Homogeneous fluoroimmunoassay of a pyrethroid metabolite in urine

Evgenia G. Matveeva, Guomin Shan, Ian M. Kennedy, Shirley J. Gee, Donald W. Stoutamire, Bruce D. Hammock

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pyrethroids are widely used in agriculture as insecticides. In this study, we describe a simple one-step homogeneous fluoroimmunoassay for the glycine conjugate of phenoxybenzoic acid (PBAG), a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Quenching fluoroimmunoassay (QFIA) is based on the competition of labeled and non-labeled pesticide for binding with antibodies and the resulting calibration curve is based on the relationship between analyte concentration and fluorescence quenching of labeled pesticide by specific antibodies. We developed a QFIA for PBAG in aqueous solution using fluorescein-labeled PBAG and polyclonal antibodies. The estimated IC50 (analyte concentration giving 50% inhibition of quenching) for PBAG was 4.5 nM. The detection limit (DL) was 0.9 nM. The dynamic range of the calibration curve was 2-50 nM. The average analytical recovery obtained by applying the method to urine samples (400- or 1000-fold urine dilution) was 85-111%. This demonstrates the QFIA to be a very simple and rapid detection method for PBAG; no washing steps and no enzyme conjugates were required.

Original languageEnglish (US)
Pages (from-to)103-117
Number of pages15
JournalAnalytica Chimica Acta
Volume444
Issue number1
DOIs
StatePublished - Oct 12 2001

Fingerprint

Fluoroimmunoassay
Pyrethrins
pyrethroid
Metabolites
urine
antibody
Quenching
metabolite
Urine
pesticide
Pesticides
Calibration
calibration
Antibodies
detection method
insecticide
biomarker
dilution
fluorescence
aqueous solution

Keywords

  • Fluorescence quenching
  • Homogeneous fluoroimmunoassay
  • Polyclonal antibodies
  • Pyrethroid metabolite
  • Urine

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Matveeva, E. G., Shan, G., Kennedy, I. M., Gee, S. J., Stoutamire, D. W., & Hammock, B. D. (2001). Homogeneous fluoroimmunoassay of a pyrethroid metabolite in urine. Analytica Chimica Acta, 444(1), 103-117. https://doi.org/10.1016/S0003-2670(01)01161-8

Homogeneous fluoroimmunoassay of a pyrethroid metabolite in urine. / Matveeva, Evgenia G.; Shan, Guomin; Kennedy, Ian M.; Gee, Shirley J.; Stoutamire, Donald W.; Hammock, Bruce D.

In: Analytica Chimica Acta, Vol. 444, No. 1, 12.10.2001, p. 103-117.

Research output: Contribution to journalArticle

Matveeva, EG, Shan, G, Kennedy, IM, Gee, SJ, Stoutamire, DW & Hammock, BD 2001, 'Homogeneous fluoroimmunoassay of a pyrethroid metabolite in urine', Analytica Chimica Acta, vol. 444, no. 1, pp. 103-117. https://doi.org/10.1016/S0003-2670(01)01161-8
Matveeva EG, Shan G, Kennedy IM, Gee SJ, Stoutamire DW, Hammock BD. Homogeneous fluoroimmunoassay of a pyrethroid metabolite in urine. Analytica Chimica Acta. 2001 Oct 12;444(1):103-117. https://doi.org/10.1016/S0003-2670(01)01161-8
Matveeva, Evgenia G. ; Shan, Guomin ; Kennedy, Ian M. ; Gee, Shirley J. ; Stoutamire, Donald W. ; Hammock, Bruce D. / Homogeneous fluoroimmunoassay of a pyrethroid metabolite in urine. In: Analytica Chimica Acta. 2001 ; Vol. 444, No. 1. pp. 103-117.
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