Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling

J. Briscoe, L. Sussel, P. Serup, Dennis Hartigan-O'Connor, T. M. Jessell, J. L R Rubenstein, J. Ericson

Research output: Contribution to journalArticle

547 Citations (Scopus)

Abstract

During vertebrate development, the specification of distinct cell types is thought to be controlled by inductive signals acting at different concentration thresholds1. The degree of receptor activation in response to these signals is a known determinant of cell fate2, but the later steps at which graded signals are converted into all-or-none distinctions in cell identity remain poorly resolved. In the ventral neural tube, motor neuron and interneuron generation depends on the graded activity of the signalling protein Sonic hedgehog (Shh)3-5. These neuronal subtypes derive from distinct progenitor cell populations that express the homeodomain proteins Nkx2.2 or Pax6 in response to graded Shh signalling6,7. In mice lacking Pax6, progenitor cells generate neurons characteristic of exposure to greater Shh activity6,8. However, Nkx2.2 expression expands dosally in Pax6 mutants, raising the possibility that Pax6 controls neuronal pattern indirectly. Here we provide evidence that Nkx2.2 has a primary role in ventral neuronal patterning. In Nkx2.2 mutants, Pax6 expression is unchanged but cells undergo a ventral-to-dorsal transformation in fate and generate motor neurons rather than interneurons. Thus, Nkx2.2 has an essential role in interpreting graded Shh signals and selecting neuronal identity.

Original languageEnglish (US)
Pages (from-to)622-627
Number of pages6
JournalNature
Volume398
Issue number6728
DOIs
StatePublished - Apr 15 1999
Externally publishedYes

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Hedgehogs
Homeobox Genes
Motor Neurons
Interneurons
Stem Cells
Hedgehog Proteins
Homeodomain Proteins
Neural Tube
Vertebrates
Neurons
Population

ASJC Scopus subject areas

  • General

Cite this

Briscoe, J., Sussel, L., Serup, P., Hartigan-O'Connor, D., Jessell, T. M., Rubenstein, J. L. R., & Ericson, J. (1999). Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. Nature, 398(6728), 622-627. https://doi.org/10.1038/19315

Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. / Briscoe, J.; Sussel, L.; Serup, P.; Hartigan-O'Connor, Dennis; Jessell, T. M.; Rubenstein, J. L R; Ericson, J.

In: Nature, Vol. 398, No. 6728, 15.04.1999, p. 622-627.

Research output: Contribution to journalArticle

Briscoe, J, Sussel, L, Serup, P, Hartigan-O'Connor, D, Jessell, TM, Rubenstein, JLR & Ericson, J 1999, 'Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling', Nature, vol. 398, no. 6728, pp. 622-627. https://doi.org/10.1038/19315
Briscoe, J. ; Sussel, L. ; Serup, P. ; Hartigan-O'Connor, Dennis ; Jessell, T. M. ; Rubenstein, J. L R ; Ericson, J. / Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. In: Nature. 1999 ; Vol. 398, No. 6728. pp. 622-627.
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