Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array

Waheb Bishara, Uzair Sikora, Onur Mudanyali, Ting Wei Su, Oguzhan Yaglidere, Shirley Luckhart, Aydogan Ozcan

Research output: Contribution to journalArticlepeer-review

195 Scopus citations

Abstract

We report a portable lensless on-chip microscope that can achieve <1 m resolution over a wide field-of-view of ∼24 mm2 without the use of any mechanical scanning. This compact on-chip microscope weighs ∼95 g and is based on partially coherent digital in-line holography. Multiple fiber-optic waveguides are butt-coupled to light emitting diodes, which are controlled by a low-cost micro-controller to sequentially illuminate the sample. The resulting lensfree holograms are then captured by a digital sensor-array and are rapidly processed using a pixel super-resolution algorithm to generate much higher resolution holographic images (both phase and amplitude) of the objects. This wide-field and high-resolution on-chip microscope, being compact and light-weight, would be important for global health problems such as diagnosis of infectious diseases in remote locations. Toward this end, we validate the performance of this field-portable microscope by imaging human malaria parasites (Plasmodium falciparum) in thin blood smears. Our results constitute the first-time that a lensfree on-chip microscope has successfully imaged malaria parasites.

Original languageEnglish (US)
Pages (from-to)1276-1279
Number of pages4
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume11
Issue number7
DOIs
StatePublished - Apr 7 2011

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

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