Abstract
We report a portable lensless on-chip microscope that can achieve <1 m resolution over a wide field-of-view of ∼24 mm2 without the use of any mechanical scanning. This compact on-chip microscope weighs ∼95 g and is based on partially coherent digital in-line holography. Multiple fiber-optic waveguides are butt-coupled to light emitting diodes, which are controlled by a low-cost micro-controller to sequentially illuminate the sample. The resulting lensfree holograms are then captured by a digital sensor-array and are rapidly processed using a pixel super-resolution algorithm to generate much higher resolution holographic images (both phase and amplitude) of the objects. This wide-field and high-resolution on-chip microscope, being compact and light-weight, would be important for global health problems such as diagnosis of infectious diseases in remote locations. Toward this end, we validate the performance of this field-portable microscope by imaging human malaria parasites (Plasmodium falciparum) in thin blood smears. Our results constitute the first-time that a lensfree on-chip microscope has successfully imaged malaria parasites.
Original language | English (US) |
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Pages (from-to) | 1276-1279 |
Number of pages | 4 |
Journal | Lab on a Chip - Miniaturisation for Chemistry and Biology |
Volume | 11 |
Issue number | 7 |
DOIs | |
State | Published - Apr 7 2011 |
ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
- Bioengineering
- Biomedical Engineering