HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300

Feng Zhou, Xiaomei Liu, Dongjiao Zuo, Min Xue, Lin Gao, Ying Yang, Jing Wang, Liping Niu, Qianwen Cao, Xiangyang Li, Hui Hua, Bo Zhang, Minmin Hu, Dianshuai Gao, Kuiyang Zheng, Yoshihiro Izumiya, Renxian Tang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: HIV-associated neurocognitive disorder (HAND) is a neurodegenerative disease associated with persistent neuroinflammation and subsequent neuron damage. Pro-inflammatory factors and neurotoxins from activated astrocytes by HIV-1 itself and its encoded proteins, including the negative factor (Nef), are involved in the pathogenesis of HAND. This study was designed to find potential lncRNAs that regulate astrocyte functions and inflammation process. Methods: We performed microarray analysis of lncRNAs from primary mouse astrocytes treated with Nef protein. Top ten lncRNAs were validated through real-time PCR analysis. Gene ontology (GO) and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. RIP and ChIP assays were performed to demonstrate the mechanism of lncRNA regulating gene expression. Results: There were 638 co-upregulated lncRNAs and 372 co-downregulated lncRNAs in primary astrocytes treated with Nef protein for both 6 h and 12 h. GO and KEGG pathway analysis showed that the biological functions of top differential-expressed mRNAs were associated with inflammatory cytokines and chemokine. Knockdown of lncRNA AK006025, not AK138360, inhibited significantly CXCL9, CXCL10 (IP-10), and CXCL11 expression in astrocytes treated with Nef protein. Mechanism study showed that AK006025 associated with CBP/P300 was enriched in the promoter of CXCL9, CXCL10, and CXCL11 genes. Conclusions: Our findings uncovered the expression profiles of lncRNAs and mRNAs in vitro, which might help to understand the pathways that regulate astrocyte activation during the process of HAND.

Original languageEnglish (US)
Article number303
JournalJournal of Neuroinflammation
Volume15
Issue number1
DOIs
StatePublished - Oct 31 2018

Fingerprint

Long Noncoding RNA
Astrocytes
HIV-1
Gene Expression
Gene Ontology
HIV
Proteins
Messenger RNA
Neurotoxins
Microarray Analysis
Chemokines
Neurodegenerative Diseases
Real-Time Polymerase Chain Reaction
Down-Regulation
Cytokines
Inflammation
Neurons

Keywords

  • Astrocytes
  • CBP/P300
  • HIV-1
  • HIV-associated neurocognitive disorder
  • Inflammation
  • lncRNAs
  • Nef

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300. / Zhou, Feng; Liu, Xiaomei; Zuo, Dongjiao; Xue, Min; Gao, Lin; Yang, Ying; Wang, Jing; Niu, Liping; Cao, Qianwen; Li, Xiangyang; Hua, Hui; Zhang, Bo; Hu, Minmin; Gao, Dianshuai; Zheng, Kuiyang; Izumiya, Yoshihiro; Tang, Renxian.

In: Journal of Neuroinflammation, Vol. 15, No. 1, 303, 31.10.2018.

Research output: Contribution to journalArticle

Zhou, F, Liu, X, Zuo, D, Xue, M, Gao, L, Yang, Y, Wang, J, Niu, L, Cao, Q, Li, X, Hua, H, Zhang, B, Hu, M, Gao, D, Zheng, K, Izumiya, Y & Tang, R 2018, 'HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300', Journal of Neuroinflammation, vol. 15, no. 1, 303. https://doi.org/10.1186/s12974-018-1343-x
Zhou, Feng ; Liu, Xiaomei ; Zuo, Dongjiao ; Xue, Min ; Gao, Lin ; Yang, Ying ; Wang, Jing ; Niu, Liping ; Cao, Qianwen ; Li, Xiangyang ; Hua, Hui ; Zhang, Bo ; Hu, Minmin ; Gao, Dianshuai ; Zheng, Kuiyang ; Izumiya, Yoshihiro ; Tang, Renxian. / HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300. In: Journal of Neuroinflammation. 2018 ; Vol. 15, No. 1.
@article{b567913c9b4444fdba23fda9e107a310,
title = "HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300",
abstract = "Background: HIV-associated neurocognitive disorder (HAND) is a neurodegenerative disease associated with persistent neuroinflammation and subsequent neuron damage. Pro-inflammatory factors and neurotoxins from activated astrocytes by HIV-1 itself and its encoded proteins, including the negative factor (Nef), are involved in the pathogenesis of HAND. This study was designed to find potential lncRNAs that regulate astrocyte functions and inflammation process. Methods: We performed microarray analysis of lncRNAs from primary mouse astrocytes treated with Nef protein. Top ten lncRNAs were validated through real-time PCR analysis. Gene ontology (GO) and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. RIP and ChIP assays were performed to demonstrate the mechanism of lncRNA regulating gene expression. Results: There were 638 co-upregulated lncRNAs and 372 co-downregulated lncRNAs in primary astrocytes treated with Nef protein for both 6 h and 12 h. GO and KEGG pathway analysis showed that the biological functions of top differential-expressed mRNAs were associated with inflammatory cytokines and chemokine. Knockdown of lncRNA AK006025, not AK138360, inhibited significantly CXCL9, CXCL10 (IP-10), and CXCL11 expression in astrocytes treated with Nef protein. Mechanism study showed that AK006025 associated with CBP/P300 was enriched in the promoter of CXCL9, CXCL10, and CXCL11 genes. Conclusions: Our findings uncovered the expression profiles of lncRNAs and mRNAs in vitro, which might help to understand the pathways that regulate astrocyte activation during the process of HAND.",
keywords = "Astrocytes, CBP/P300, HIV-1, HIV-associated neurocognitive disorder, Inflammation, lncRNAs, Nef",
author = "Feng Zhou and Xiaomei Liu and Dongjiao Zuo and Min Xue and Lin Gao and Ying Yang and Jing Wang and Liping Niu and Qianwen Cao and Xiangyang Li and Hui Hua and Bo Zhang and Minmin Hu and Dianshuai Gao and Kuiyang Zheng and Yoshihiro Izumiya and Renxian Tang",
year = "2018",
month = "10",
day = "31",
doi = "10.1186/s12974-018-1343-x",
language = "English (US)",
volume = "15",
journal = "Journal of Neuroinflammation",
issn = "1742-2094",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300

AU - Zhou, Feng

AU - Liu, Xiaomei

AU - Zuo, Dongjiao

AU - Xue, Min

AU - Gao, Lin

AU - Yang, Ying

AU - Wang, Jing

AU - Niu, Liping

AU - Cao, Qianwen

AU - Li, Xiangyang

AU - Hua, Hui

AU - Zhang, Bo

AU - Hu, Minmin

AU - Gao, Dianshuai

AU - Zheng, Kuiyang

AU - Izumiya, Yoshihiro

AU - Tang, Renxian

PY - 2018/10/31

Y1 - 2018/10/31

N2 - Background: HIV-associated neurocognitive disorder (HAND) is a neurodegenerative disease associated with persistent neuroinflammation and subsequent neuron damage. Pro-inflammatory factors and neurotoxins from activated astrocytes by HIV-1 itself and its encoded proteins, including the negative factor (Nef), are involved in the pathogenesis of HAND. This study was designed to find potential lncRNAs that regulate astrocyte functions and inflammation process. Methods: We performed microarray analysis of lncRNAs from primary mouse astrocytes treated with Nef protein. Top ten lncRNAs were validated through real-time PCR analysis. Gene ontology (GO) and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. RIP and ChIP assays were performed to demonstrate the mechanism of lncRNA regulating gene expression. Results: There were 638 co-upregulated lncRNAs and 372 co-downregulated lncRNAs in primary astrocytes treated with Nef protein for both 6 h and 12 h. GO and KEGG pathway analysis showed that the biological functions of top differential-expressed mRNAs were associated with inflammatory cytokines and chemokine. Knockdown of lncRNA AK006025, not AK138360, inhibited significantly CXCL9, CXCL10 (IP-10), and CXCL11 expression in astrocytes treated with Nef protein. Mechanism study showed that AK006025 associated with CBP/P300 was enriched in the promoter of CXCL9, CXCL10, and CXCL11 genes. Conclusions: Our findings uncovered the expression profiles of lncRNAs and mRNAs in vitro, which might help to understand the pathways that regulate astrocyte activation during the process of HAND.

AB - Background: HIV-associated neurocognitive disorder (HAND) is a neurodegenerative disease associated with persistent neuroinflammation and subsequent neuron damage. Pro-inflammatory factors and neurotoxins from activated astrocytes by HIV-1 itself and its encoded proteins, including the negative factor (Nef), are involved in the pathogenesis of HAND. This study was designed to find potential lncRNAs that regulate astrocyte functions and inflammation process. Methods: We performed microarray analysis of lncRNAs from primary mouse astrocytes treated with Nef protein. Top ten lncRNAs were validated through real-time PCR analysis. Gene ontology (GO) and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. RIP and ChIP assays were performed to demonstrate the mechanism of lncRNA regulating gene expression. Results: There were 638 co-upregulated lncRNAs and 372 co-downregulated lncRNAs in primary astrocytes treated with Nef protein for both 6 h and 12 h. GO and KEGG pathway analysis showed that the biological functions of top differential-expressed mRNAs were associated with inflammatory cytokines and chemokine. Knockdown of lncRNA AK006025, not AK138360, inhibited significantly CXCL9, CXCL10 (IP-10), and CXCL11 expression in astrocytes treated with Nef protein. Mechanism study showed that AK006025 associated with CBP/P300 was enriched in the promoter of CXCL9, CXCL10, and CXCL11 genes. Conclusions: Our findings uncovered the expression profiles of lncRNAs and mRNAs in vitro, which might help to understand the pathways that regulate astrocyte activation during the process of HAND.

KW - Astrocytes

KW - CBP/P300

KW - HIV-1

KW - HIV-associated neurocognitive disorder

KW - Inflammation

KW - lncRNAs

KW - Nef

UR - http://www.scopus.com/inward/record.url?scp=85055770873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055770873&partnerID=8YFLogxK

U2 - 10.1186/s12974-018-1343-x

DO - 10.1186/s12974-018-1343-x

M3 - Article

C2 - 30382871

AN - SCOPUS:85055770873

VL - 15

JO - Journal of Neuroinflammation

JF - Journal of Neuroinflammation

SN - 1742-2094

IS - 1

M1 - 303

ER -