HIV-1 drug resistance in variants from the female genital tract and plasma

Kimdar Sherefa Kemal, Harold Burger, Douglas Mayers, Kathryn Anastos, Brian Foley, Christina Kitchen, Penelope Huggins, Tamara Schroeder, Gaston Picchio, Sara Back, Wei Gao, William A. Meyer, Barbara Weiser

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background. Human immunodeficiency virus type 1 (HIV-1) drug-resistance mutations may arise in a fraction of viral variants, and these variants may differ between compartments, including the genital tract and blood. Methods. We studied 14 women with detectable HIV-1 in both the genital tract and plasma despite antiretroviral treatment. We obtained HIV-1 RNA sequences from 280 unique viral variants and then determined the resistance genotype and the predicted phenotype (Virtual Phenotype; Virco BVBA) of each variant. Results. Eight patients (57%) displayed mutations conferring high-level HIV-1 drug resistance. Although we observed differences in specific mutations among viral variants, 13 of the 14 women showed highly concordant HIV-1 genotypic and predicted phenotypic resistance patterns in the 2 compartments. In 1 patient, resistance mutations appeared only in plasma; all variants in her genital tract, which displayed a low viral load, were susceptible. Conclusions. These data suggest that, for the majority of women, determination of HIV-1 drug resistance in the plasma will approximate the drug-resistance pattern in the genital tract. Analysis of individual variants enabled us to identify minority species bearing distinctive linked mutations, which may serve as a source of novel resistance genotypes. These data are relevant to clinical management and the evolution of drug resistance.

Original languageEnglish (US)
Pages (from-to)535-545
Number of pages11
JournalJournal of Infectious Diseases
Volume195
Issue number4
DOIs
StatePublished - Feb 15 2007
Externally publishedYes

Fingerprint

Drug Resistance
HIV-1
Mutation
Genotype
Phenotype
Viral Load

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

HIV-1 drug resistance in variants from the female genital tract and plasma. / Kemal, Kimdar Sherefa; Burger, Harold; Mayers, Douglas; Anastos, Kathryn; Foley, Brian; Kitchen, Christina; Huggins, Penelope; Schroeder, Tamara; Picchio, Gaston; Back, Sara; Gao, Wei; Meyer, William A.; Weiser, Barbara.

In: Journal of Infectious Diseases, Vol. 195, No. 4, 15.02.2007, p. 535-545.

Research output: Contribution to journalArticle

Kemal, KS, Burger, H, Mayers, D, Anastos, K, Foley, B, Kitchen, C, Huggins, P, Schroeder, T, Picchio, G, Back, S, Gao, W, Meyer, WA & Weiser, B 2007, 'HIV-1 drug resistance in variants from the female genital tract and plasma', Journal of Infectious Diseases, vol. 195, no. 4, pp. 535-545. https://doi.org/10.1086/510855
Kemal, Kimdar Sherefa ; Burger, Harold ; Mayers, Douglas ; Anastos, Kathryn ; Foley, Brian ; Kitchen, Christina ; Huggins, Penelope ; Schroeder, Tamara ; Picchio, Gaston ; Back, Sara ; Gao, Wei ; Meyer, William A. ; Weiser, Barbara. / HIV-1 drug resistance in variants from the female genital tract and plasma. In: Journal of Infectious Diseases. 2007 ; Vol. 195, No. 4. pp. 535-545.
@article{498091626acf47e3bf0aade83fa8633d,
title = "HIV-1 drug resistance in variants from the female genital tract and plasma",
abstract = "Background. Human immunodeficiency virus type 1 (HIV-1) drug-resistance mutations may arise in a fraction of viral variants, and these variants may differ between compartments, including the genital tract and blood. Methods. We studied 14 women with detectable HIV-1 in both the genital tract and plasma despite antiretroviral treatment. We obtained HIV-1 RNA sequences from 280 unique viral variants and then determined the resistance genotype and the predicted phenotype (Virtual Phenotype; Virco BVBA) of each variant. Results. Eight patients (57{\%}) displayed mutations conferring high-level HIV-1 drug resistance. Although we observed differences in specific mutations among viral variants, 13 of the 14 women showed highly concordant HIV-1 genotypic and predicted phenotypic resistance patterns in the 2 compartments. In 1 patient, resistance mutations appeared only in plasma; all variants in her genital tract, which displayed a low viral load, were susceptible. Conclusions. These data suggest that, for the majority of women, determination of HIV-1 drug resistance in the plasma will approximate the drug-resistance pattern in the genital tract. Analysis of individual variants enabled us to identify minority species bearing distinctive linked mutations, which may serve as a source of novel resistance genotypes. These data are relevant to clinical management and the evolution of drug resistance.",
author = "Kemal, {Kimdar Sherefa} and Harold Burger and Douglas Mayers and Kathryn Anastos and Brian Foley and Christina Kitchen and Penelope Huggins and Tamara Schroeder and Gaston Picchio and Sara Back and Wei Gao and Meyer, {William A.} and Barbara Weiser",
year = "2007",
month = "2",
day = "15",
doi = "10.1086/510855",
language = "English (US)",
volume = "195",
pages = "535--545",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - HIV-1 drug resistance in variants from the female genital tract and plasma

AU - Kemal, Kimdar Sherefa

AU - Burger, Harold

AU - Mayers, Douglas

AU - Anastos, Kathryn

AU - Foley, Brian

AU - Kitchen, Christina

AU - Huggins, Penelope

AU - Schroeder, Tamara

AU - Picchio, Gaston

AU - Back, Sara

AU - Gao, Wei

AU - Meyer, William A.

AU - Weiser, Barbara

PY - 2007/2/15

Y1 - 2007/2/15

N2 - Background. Human immunodeficiency virus type 1 (HIV-1) drug-resistance mutations may arise in a fraction of viral variants, and these variants may differ between compartments, including the genital tract and blood. Methods. We studied 14 women with detectable HIV-1 in both the genital tract and plasma despite antiretroviral treatment. We obtained HIV-1 RNA sequences from 280 unique viral variants and then determined the resistance genotype and the predicted phenotype (Virtual Phenotype; Virco BVBA) of each variant. Results. Eight patients (57%) displayed mutations conferring high-level HIV-1 drug resistance. Although we observed differences in specific mutations among viral variants, 13 of the 14 women showed highly concordant HIV-1 genotypic and predicted phenotypic resistance patterns in the 2 compartments. In 1 patient, resistance mutations appeared only in plasma; all variants in her genital tract, which displayed a low viral load, were susceptible. Conclusions. These data suggest that, for the majority of women, determination of HIV-1 drug resistance in the plasma will approximate the drug-resistance pattern in the genital tract. Analysis of individual variants enabled us to identify minority species bearing distinctive linked mutations, which may serve as a source of novel resistance genotypes. These data are relevant to clinical management and the evolution of drug resistance.

AB - Background. Human immunodeficiency virus type 1 (HIV-1) drug-resistance mutations may arise in a fraction of viral variants, and these variants may differ between compartments, including the genital tract and blood. Methods. We studied 14 women with detectable HIV-1 in both the genital tract and plasma despite antiretroviral treatment. We obtained HIV-1 RNA sequences from 280 unique viral variants and then determined the resistance genotype and the predicted phenotype (Virtual Phenotype; Virco BVBA) of each variant. Results. Eight patients (57%) displayed mutations conferring high-level HIV-1 drug resistance. Although we observed differences in specific mutations among viral variants, 13 of the 14 women showed highly concordant HIV-1 genotypic and predicted phenotypic resistance patterns in the 2 compartments. In 1 patient, resistance mutations appeared only in plasma; all variants in her genital tract, which displayed a low viral load, were susceptible. Conclusions. These data suggest that, for the majority of women, determination of HIV-1 drug resistance in the plasma will approximate the drug-resistance pattern in the genital tract. Analysis of individual variants enabled us to identify minority species bearing distinctive linked mutations, which may serve as a source of novel resistance genotypes. These data are relevant to clinical management and the evolution of drug resistance.

UR - http://www.scopus.com/inward/record.url?scp=33846784099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846784099&partnerID=8YFLogxK

U2 - 10.1086/510855

DO - 10.1086/510855

M3 - Article

C2 - 17230413

AN - SCOPUS:33846784099

VL - 195

SP - 535

EP - 545

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 4

ER -