Abstract
Three types of prosthetic anterior cruciate ligaments were investigated by enzymatic and histological analysis of the tissue surrounding each implant and immunologically by a cytofluorometric analysis of T-cell populations in the peripheral blood of rats. Two of the prostheses had a braided construction, one made from polyester and the other from high performance polyethylene fibres. The third type also contained high performance polyethylene fibres, but had been manufactured in a knitted construction (Raschel high performance polyethylene). Five specimens from each prosthesis were implanted intraperitoneally in rats by a trocar for different periods of time up to 4 wk. A control group of rats underwent the surgery, but not the implant. No modification in peripheral T-cell populations was induced by the presence of any implant. Whilst the levels of acid phosphatase and esterase activity appeared to have increased slightly following implantation of any of the prostheses, such increases were not highly significant. Histologically, all three materials induced an intense acute inflammatory reaction at 3 d which gave way to a typical chronic response after 4 wk. The only major difference between the prostheses was that after 4 wk the polyester fibres exhibited less inflammation, and the surrounding tissue was more mature, more vascularized and more densely infiltrated with collagen than with the two high performance polyethylene implants. In conclusion, all three devices provided satisfactory biocompatibility in terms of cellular and healing response.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 255-262 |
| Number of pages | 8 |
| Journal | Biomaterials |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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Keywords
- acid phosphatase
- polyethylene
- Prosthetic ligaments
- T-cell populations
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Histopathological and immunological investigations of synthetic fibres and structures used in three prosthetic anterior cruciate ligaments : in vivo study in the rat. / Marois, Yves; Roy, Raynald; Vidovszky, Tamas J; King, Martin W.; Bélanger, Alain Y.; Chaput, Cyril; Guidoin, Robert.
In: Biomaterials, Vol. 14, No. 4, 1993, p. 255-262.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Histopathological and immunological investigations of synthetic fibres and structures used in three prosthetic anterior cruciate ligaments
T2 - in vivo study in the rat
AU - Marois, Yves
AU - Roy, Raynald
AU - Vidovszky, Tamas J
AU - King, Martin W.
AU - Bélanger, Alain Y.
AU - Chaput, Cyril
AU - Guidoin, Robert
PY - 1993
Y1 - 1993
N2 - Three types of prosthetic anterior cruciate ligaments were investigated by enzymatic and histological analysis of the tissue surrounding each implant and immunologically by a cytofluorometric analysis of T-cell populations in the peripheral blood of rats. Two of the prostheses had a braided construction, one made from polyester and the other from high performance polyethylene fibres. The third type also contained high performance polyethylene fibres, but had been manufactured in a knitted construction (Raschel high performance polyethylene). Five specimens from each prosthesis were implanted intraperitoneally in rats by a trocar for different periods of time up to 4 wk. A control group of rats underwent the surgery, but not the implant. No modification in peripheral T-cell populations was induced by the presence of any implant. Whilst the levels of acid phosphatase and esterase activity appeared to have increased slightly following implantation of any of the prostheses, such increases were not highly significant. Histologically, all three materials induced an intense acute inflammatory reaction at 3 d which gave way to a typical chronic response after 4 wk. The only major difference between the prostheses was that after 4 wk the polyester fibres exhibited less inflammation, and the surrounding tissue was more mature, more vascularized and more densely infiltrated with collagen than with the two high performance polyethylene implants. In conclusion, all three devices provided satisfactory biocompatibility in terms of cellular and healing response.
AB - Three types of prosthetic anterior cruciate ligaments were investigated by enzymatic and histological analysis of the tissue surrounding each implant and immunologically by a cytofluorometric analysis of T-cell populations in the peripheral blood of rats. Two of the prostheses had a braided construction, one made from polyester and the other from high performance polyethylene fibres. The third type also contained high performance polyethylene fibres, but had been manufactured in a knitted construction (Raschel high performance polyethylene). Five specimens from each prosthesis were implanted intraperitoneally in rats by a trocar for different periods of time up to 4 wk. A control group of rats underwent the surgery, but not the implant. No modification in peripheral T-cell populations was induced by the presence of any implant. Whilst the levels of acid phosphatase and esterase activity appeared to have increased slightly following implantation of any of the prostheses, such increases were not highly significant. Histologically, all three materials induced an intense acute inflammatory reaction at 3 d which gave way to a typical chronic response after 4 wk. The only major difference between the prostheses was that after 4 wk the polyester fibres exhibited less inflammation, and the surrounding tissue was more mature, more vascularized and more densely infiltrated with collagen than with the two high performance polyethylene implants. In conclusion, all three devices provided satisfactory biocompatibility in terms of cellular and healing response.
KW - acid phosphatase
KW - polyethylene
KW - Prosthetic ligaments
KW - T-cell populations
UR - http://www.scopus.com/inward/record.url?scp=0027571723&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027571723&partnerID=8YFLogxK
U2 - 10.1016/0142-9612(93)90115-I
DO - 10.1016/0142-9612(93)90115-I
M3 - Article
C2 - 8476995
AN - SCOPUS:0027571723
VL - 14
SP - 255
EP - 262
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 4
ER -