Histologic and molecular correlation in shelter cats with acute upper respiratory infection

Rachel E. Burns, Denae C. Wagner, Christian M. Leutenegger, Patricia Pesavento

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This is a descriptive study designed to correlate diagnostic real-time PCR results with histopathologic lesions in cats with clinical signs of upper respiratory infection (URI). The study occurred over a 9-month period in a single open-intake animal shelter. Cats that were selected for euthanasia by the shelter staff and additionally had URI were included in the study, for a total of 22 study cats. Combined conjunctival and oropharyngeal swab specimens were tested by quantitative real-time PCR (qPCR) for feline herpesvirus type 1 (FHV-1), feline calicivirus (FCV), Mycoplasma felis, Chlamydophila felis, and Bordetella bronchiseptica. Necropsy was performed on all cats, and a complete set of respiratory tract tissues was examined by histopathology. Among 22 cats, 20 were qPCR positive for FHV-1, 7 for M. felis, 5 for FCV, 1 for C. felis, and 0 for B. bronchiseptica. Nine cats were positive for two or more pathogens. Histopathologic lesions were present in all cats, with consistent lesions in the nasal cavity, including acute necroulcerative rhinitis in 16 cats. Histologic or antigenic detection of FHV-1 was seen in 18 of 20 cats positive for FHV-1 by qPCR. No lesions that could be specifically attributed to FCV, M. felis, or C. felis were seen, although interpretation in this cohort could be confounded by coinfection with FHV-1. A significant agreement was found between the amount of FHV-1 DNA determined by qPCR and the presence of specific histopathologic lesions for FHV-1 but not for the other respiratory pathogens.

Original languageEnglish (US)
Pages (from-to)2454-2460
Number of pages7
JournalJournal of Clinical Microbiology
Volume49
Issue number7
DOIs
StatePublished - Jul 2011

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Felis
Respiratory Tract Infections
Herpesviridae
Felidae
Cats
Feline Calicivirus
Bordetella bronchiseptica
Real-Time Polymerase Chain Reaction
Chlamydophila
Euthanasia
Mycoplasma
Nasal Cavity
Rhinitis
Coinfection
Respiratory System
DNA

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Histologic and molecular correlation in shelter cats with acute upper respiratory infection. / Burns, Rachel E.; Wagner, Denae C.; Leutenegger, Christian M.; Pesavento, Patricia.

In: Journal of Clinical Microbiology, Vol. 49, No. 7, 07.2011, p. 2454-2460.

Research output: Contribution to journalArticle

Burns, Rachel E. ; Wagner, Denae C. ; Leutenegger, Christian M. ; Pesavento, Patricia. / Histologic and molecular correlation in shelter cats with acute upper respiratory infection. In: Journal of Clinical Microbiology. 2011 ; Vol. 49, No. 7. pp. 2454-2460.
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