Histidine mutants requiring adenine: selection of mutants with reduced hisG expression in Salmonella typhimurium.

H. M. Johnston, J. R. Roth

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A method is described for the selection of Salmonella typhimurium mutants with reduced levels of hisG enzyme activity. This method is based on the fact that the hisG enzyme catalyzes the consumption of ATP in the first step of histidine biosynthesis. Normally, this reaction is closely regulated, both by feedback inhibition and by repression of the operon. However, conditions can be set up that result in the uncontrolled use of adenine in histidine biosynthesis. Cells grown under these conditions become phenotypic adenine auxotrophs. Some revertant clones that no longer require adenine contain mutations in hisG, hisE, or the his-control region. The hisG mutations are of all types (nonsense, frameshift, missense, deletion and leady types), and they map throughout the hisG gene.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalGenetics
Volume92
Issue number1
StatePublished - May 1979
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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