Three calves were sensitized with three doses of inactivated BTV-11 UC8 strain and then experimentally infected with the homologous virus. In addition, four BTV-seronegative heifers were also experimentally infected with BTV-11. Granulocyte rich fractions of peripheral blood leucocyte (PBL-GRF) cultures from BTV-sensitized/infected calves and from control unexposed cattle were exposed in vitro with BTV-11. Histamine, leukotriene (LT) C 4 and prostaglandin (PG) D 2 were assayed in supernatant fluids. Plasma histamine levels increased in BTV-infected heifers from 10.1 ± 2 ng/ml at Day 0 to 23.1 ± 6.6 ng/ml at Day 12 following virus exposure. In addition, in this experimental group the concentration of PGF 2α, (mean 551.97 ± 243.54 pg/ml) increased significantly (P ≤ 0.05) compared with control cattle (mean 467.3 ± 73.9pg/ml). Bluetongue virus induced histamine and LTC 4 release after in vitro infection of PBL-GRF. Release of LTC 4 was significantly (P ≤ 0.05) higher in PBL-GRF cultures from sensitized and control animals than in unexposed PBL-GRF cultures. In contrast to these results, PGD 2 was not released after BTV infection of PBL-GRF in vitro. The histamine release caused by BTV was virus-specific and mainly mediated by an immunological reaction, since the release was significantly reduced by removal of cell surface immunoglobulins.
|Original language||English (US)|
|Number of pages||12|
|Journal||Journal of Veterinary Medicine, Series B|
|State||Published - Oct 1 1997|
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