TY - JOUR
T1 - Histamine and Eicosanoid Levels in Plasma and Peripheral Blood Leucocyte Cultures during Experimental Infection of Cattle with Bluetongue Virus Serotype 11
AU - Odeón, A. C.
AU - Giri, S. N.
AU - De La Concha-Bermejillo, A.
AU - Osburn, Bennie
PY - 1997/10/1
Y1 - 1997/10/1
N2 - Three calves were sensitized with three doses of inactivated BTV-11 UC8 strain and then experimentally infected with the homologous virus. In addition, four BTV-seronegative heifers were also experimentally infected with BTV-11. Granulocyte rich fractions of peripheral blood leucocyte (PBL-GRF) cultures from BTV-sensitized/infected calves and from control unexposed cattle were exposed in vitro with BTV-11. Histamine, leukotriene (LT) C 4 and prostaglandin (PG) D 2 were assayed in supernatant fluids. Plasma histamine levels increased in BTV-infected heifers from 10.1 ± 2 ng/ml at Day 0 to 23.1 ± 6.6 ng/ml at Day 12 following virus exposure. In addition, in this experimental group the concentration of PGF 2α, (mean 551.97 ± 243.54 pg/ml) increased significantly (P ≤ 0.05) compared with control cattle (mean 467.3 ± 73.9pg/ml). Bluetongue virus induced histamine and LTC 4 release after in vitro infection of PBL-GRF. Release of LTC 4 was significantly (P ≤ 0.05) higher in PBL-GRF cultures from sensitized and control animals than in unexposed PBL-GRF cultures. In contrast to these results, PGD 2 was not released after BTV infection of PBL-GRF in vitro. The histamine release caused by BTV was virus-specific and mainly mediated by an immunological reaction, since the release was significantly reduced by removal of cell surface immunoglobulins.
AB - Three calves were sensitized with three doses of inactivated BTV-11 UC8 strain and then experimentally infected with the homologous virus. In addition, four BTV-seronegative heifers were also experimentally infected with BTV-11. Granulocyte rich fractions of peripheral blood leucocyte (PBL-GRF) cultures from BTV-sensitized/infected calves and from control unexposed cattle were exposed in vitro with BTV-11. Histamine, leukotriene (LT) C 4 and prostaglandin (PG) D 2 were assayed in supernatant fluids. Plasma histamine levels increased in BTV-infected heifers from 10.1 ± 2 ng/ml at Day 0 to 23.1 ± 6.6 ng/ml at Day 12 following virus exposure. In addition, in this experimental group the concentration of PGF 2α, (mean 551.97 ± 243.54 pg/ml) increased significantly (P ≤ 0.05) compared with control cattle (mean 467.3 ± 73.9pg/ml). Bluetongue virus induced histamine and LTC 4 release after in vitro infection of PBL-GRF. Release of LTC 4 was significantly (P ≤ 0.05) higher in PBL-GRF cultures from sensitized and control animals than in unexposed PBL-GRF cultures. In contrast to these results, PGD 2 was not released after BTV infection of PBL-GRF in vitro. The histamine release caused by BTV was virus-specific and mainly mediated by an immunological reaction, since the release was significantly reduced by removal of cell surface immunoglobulins.
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M3 - Article
C2 - 9394610
AN - SCOPUS:0031241651
VL - 44
SP - 449
EP - 460
JO - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health
JF - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health
SN - 1863-1959
IS - 8
ER -