Highly tissue substructure-specific effects of human papilloma virus in mucosa of HIV-infected patients revealed by laser-dissection microscopy-assisted gene expression profiling

Nicole Baumgarth, Richard Szubin, Greg M. Dolganov, Mitchell R. Watnik, Deborah Greenspan, Maria Da Costa, Joel M. Palefsky, Richard Jordan, Mario Roederer, John S. Greenspan

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Human papilloma virus (HPV) causes focal infections of epithelial layers in skin and mucosa. HIV-infected patients on highly active antiretroviral therapy (HAART) appear to be at increased risk of developing HFV-induced oral warts. To identify the mechanisms that allow long-term infection of oral epithelial cells in these patients, we used a combination of laser-dissection microscopy (LDM) and highly sensitive and quantitative, non-biased, two-step multiplex real-time RT-PCR to study pathogen-induced alterations of specific tissue subcompartments. Expression of 166 genes was compared in three distinct epithelial and subepithelial compartments isolated from biopsies of normal mucosa from HIV-infected and non-infected patients and of HPV32-induced oral warts from HIV-infected patients. In contrast to title underlying HIV infection and/or HAART, which did not significantly elaborate tissue substructure-specific effects, changes in oral warts were strongly tissue substructure-specific. HPV 32 seems to establish infection by selectively enhancing epithelial cell growth and differentiation in the stratum spinosum and to evade the immune system by actively suppressing inflammatory responses in adjacent underlying tissues. With this highly sensitive and quantitative method tissue-specific expression of hundreds of genes can be studied simultaneously in a few cells. Because of its large dynamic measurement range it could also become a method of choice to confirm and better quantify results obtained by microarray analysis.

Original languageEnglish (US)
Pages (from-to)707-718
Number of pages12
JournalAmerican Journal of Pathology
Volume165
Issue number3
StatePublished - Sep 2004

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Papillomaviridae
Gene Expression Profiling
Confocal Microscopy
Dissection
Mucous Membrane
Warts
HIV
Highly Active Antiretroviral Therapy
Epithelial Cells
Focal Infection
Gene Expression
Multiplex Polymerase Chain Reaction
Microarray Analysis
Infection
HIV Infections
Real-Time Polymerase Chain Reaction
Cell Differentiation
Immune System
Biopsy
Skin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Highly tissue substructure-specific effects of human papilloma virus in mucosa of HIV-infected patients revealed by laser-dissection microscopy-assisted gene expression profiling. / Baumgarth, Nicole; Szubin, Richard; Dolganov, Greg M.; Watnik, Mitchell R.; Greenspan, Deborah; Da Costa, Maria; Palefsky, Joel M.; Jordan, Richard; Roederer, Mario; Greenspan, John S.

In: American Journal of Pathology, Vol. 165, No. 3, 09.2004, p. 707-718.

Research output: Contribution to journalArticle

Baumgarth, N, Szubin, R, Dolganov, GM, Watnik, MR, Greenspan, D, Da Costa, M, Palefsky, JM, Jordan, R, Roederer, M & Greenspan, JS 2004, 'Highly tissue substructure-specific effects of human papilloma virus in mucosa of HIV-infected patients revealed by laser-dissection microscopy-assisted gene expression profiling', American Journal of Pathology, vol. 165, no. 3, pp. 707-718.
Baumgarth, Nicole ; Szubin, Richard ; Dolganov, Greg M. ; Watnik, Mitchell R. ; Greenspan, Deborah ; Da Costa, Maria ; Palefsky, Joel M. ; Jordan, Richard ; Roederer, Mario ; Greenspan, John S. / Highly tissue substructure-specific effects of human papilloma virus in mucosa of HIV-infected patients revealed by laser-dissection microscopy-assisted gene expression profiling. In: American Journal of Pathology. 2004 ; Vol. 165, No. 3. pp. 707-718.
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