Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification

Joel Hwang, Hai Yu, Hamed Malekan, Go Sugiarto, Yanhong Li, Jingyao Qu, Van Nguyen, Dongyuan Wu, Xi Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Oligo(ethylene glycol)-linked light fluorous tags have been found to be optimal for conjugating to glycans for both high-yield enzymatic glycosylation reactions using one-pot multienzyme (OPME) systems and quick product purification using fluorous solid-phase extraction (FSPE) cartridges. The combination of OPME glycosylation systems and the FSPE cartridge purification scheme provides a highly effective strategy for facile synthesis and purification of glycans.

Original languageEnglish (US)
Pages (from-to)3159-3162
Number of pages4
JournalChemical Communications
Volume50
Issue number24
DOIs
StatePublished - Mar 25 2014

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Glycosylation
Purification
Polysaccharides
Ethylene Glycol
Ethylene glycol

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Cite this

Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification. / Hwang, Joel; Yu, Hai; Malekan, Hamed; Sugiarto, Go; Li, Yanhong; Qu, Jingyao; Nguyen, Van; Wu, Dongyuan; Chen, Xi.

In: Chemical Communications, Vol. 50, No. 24, 25.03.2014, p. 3159-3162.

Research output: Contribution to journalArticle

Hwang, J, Yu, H, Malekan, H, Sugiarto, G, Li, Y, Qu, J, Nguyen, V, Wu, D & Chen, X 2014, 'Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification', Chemical Communications, vol. 50, no. 24, pp. 3159-3162. https://doi.org/10.1039/c4cc00070f
Hwang, Joel ; Yu, Hai ; Malekan, Hamed ; Sugiarto, Go ; Li, Yanhong ; Qu, Jingyao ; Nguyen, Van ; Wu, Dongyuan ; Chen, Xi. / Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification. In: Chemical Communications. 2014 ; Vol. 50, No. 24. pp. 3159-3162.
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