Highly efficient monolithic silica capillary columns modified with poly(acrylic acid) for hydrophilic interaction chromatography

Kanta Horie, Tohru Ikegami, Ken Hosoya, Nabil Saad, Oliver Fiehn, Nobuo Tanaka

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Monolithic silica capillary columns for hydrophilic interaction liquid chromatography (HILIC) were prepared by on-column polymerization of acrylic acid on monolithic silica in a fused silica capillary modified with anchor groups. The products maintained the high permeability (K = 5 × 10-14 m2) and provided a plate height (H) of less than 10 μm at optimum linear velocity (u) and H below 20 μm at u = 6 mm/s for polar solutes including nucleosides and carbohydrates. The HILIC mode monolithic silica capillary column was able to produce 10 000 theoretical plates (N) with column dead time (t0) of 20 s at a pressure drop of 20 MPa or lower. The total performance was much higher than conventional particle-packed HILIC columns currently available. The gradient separations of peptides by a capillary LC-electrospray mass spectrometry system resulted in very different retention selectivity between reversed-phase mode separations and the HILIC mode separations with a peak capacity of ca. 100 in a 10 min gradient time in either mode. The high performance observed with the monolithic silica capillary column modified with poly(acrylic acid) suggests that the HILIC mode can be an alternative to the reversed-phase mode for a wide range of compounds, especially for those of high polarity in isocratic as well as gradient elution.

Original languageEnglish (US)
Pages (from-to)198-205
Number of pages8
JournalJournal of Chromatography A
Volume1164
Issue number1-2
DOIs
StatePublished - Sep 14 2007

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carbopol 940
Liquid chromatography
Chromatography
Hydrophobic and Hydrophilic Interactions
Liquid Chromatography
Silicon Dioxide
Fused silica
Anchors
Nucleosides
Polymerization
Pressure drop
Mass spectrometry
Permeability
Mass Spectrometry
Carbohydrates
Pressure
Peptides

Keywords

  • Carbohydrates
  • HILIC
  • Monolithic silica capillary columns
  • Nucleosides
  • Orthogonal selectivity
  • Peptides

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Highly efficient monolithic silica capillary columns modified with poly(acrylic acid) for hydrophilic interaction chromatography. / Horie, Kanta; Ikegami, Tohru; Hosoya, Ken; Saad, Nabil; Fiehn, Oliver; Tanaka, Nobuo.

In: Journal of Chromatography A, Vol. 1164, No. 1-2, 14.09.2007, p. 198-205.

Research output: Contribution to journalArticle

Horie, Kanta ; Ikegami, Tohru ; Hosoya, Ken ; Saad, Nabil ; Fiehn, Oliver ; Tanaka, Nobuo. / Highly efficient monolithic silica capillary columns modified with poly(acrylic acid) for hydrophilic interaction chromatography. In: Journal of Chromatography A. 2007 ; Vol. 1164, No. 1-2. pp. 198-205.
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