Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

Kam Lau; Vireak Thon; Hai Yu; Li Ding; Yi Chen; Musleh M. Muthana; Denton Wong; Ronald Huang; Xi Chen

doi:10.1039/c0cc01381a

Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

Kam Lau, Vireak Thon, Hai Yu, Li Ding, Yi Chen, Musleh M. Muthana, Denton Wong, Ronald Huang, Xi Chen

Chemistry

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.

Original language	English (US)
Pages (from-to)	6066-6068
Number of pages	3
Journal	Chemical Communications
Volume	46
Issue number	33
DOIs	https://doi.org/10.1039/c0cc01381a
State	Published - Sep 7 2010

ASJC Scopus subject areas

Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Catalysis
Chemistry(all)

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10.1039/c0cc01381a

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Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases. / Lau, Kam; Thon, Vireak; Yu, Hai; Ding, Li; Chen, Yi; Muthana, Musleh M.; Wong, Denton; Huang, Ronald; Chen, Xi.

In: Chemical Communications, Vol. 46, No. 33, 07.09.2010, p. 6066-6068.

Research output: Contribution to journal › Article › peer-review

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abstract = "Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.",

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AU - Thon, Vireak

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AU - Ding, Li

AU - Chen, Yi

AU - Muthana, Musleh M.

AU - Wong, Denton

AU - Huang, Ronald

AU - Chen, Xi

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AB - Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.

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Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

Abstract

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