Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

Kam Lau, Vireak Thon, Hai Yu, Li Ding, Yi Chen, Musleh M. Muthana, Denton Wong, Ronald Huang, Xi Chen

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.

Original languageEnglish (US)
Pages (from-to)6066-6068
Number of pages3
JournalChemical Communications
Volume46
Issue number33
DOIs
StatePublished - Sep 7 2010

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases'. Together they form a unique fingerprint.

Cite this