Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

Kam Lau, Vireak Thon, Hai Yu, Li Ding, Yi Chen, Musleh M. Muthana, Denton Wong, Ronald Huang, Xi Chen

Research output: Contribution to journal › Article

65 Citations (Scopus)

Abstract

Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.

Original languageEnglish (US)
Pages (from-to)6066-6068
Number of pages3
JournalChemical Communications
Volume46
Issue number33
DOIs
StatePublished - Sep 7 2010

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Galactosyltransferases
Galactosides
Derivatives
Monosaccharides
Lactose
Substrates

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Cite this

Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases. / Lau, Kam; Thon, Vireak; Yu, Hai; Ding, Li; Chen, Yi; Muthana, Musleh M.; Wong, Denton; Huang, Ronald; Chen, Xi.

In: Chemical Communications, Vol. 46, No. 33, 07.09.2010, p. 6066-6068.

Research output: Contribution to journal › Article

Lau, K, Thon, V, Yu, H, Ding, L, Chen, Y, Muthana, MM, Wong, D, Huang, R & Chen, X 2010, 'Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases', Chemical Communications, vol. 46, no. 33, pp. 6066-6068. https://doi.org/10.1039/c0cc01381a
Lau K, Thon V, Yu H, Ding L, Chen Y, Muthana MM et al. Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases. Chemical Communications. 2010 Sep 7;46(33):6066-6068. https://doi.org/10.1039/c0cc01381a
Lau, Kam ; Thon, Vireak ; Yu, Hai ; Ding, Li ; Chen, Yi ; Muthana, Musleh M. ; Wong, Denton ; Huang, Ronald ; Chen, Xi. / Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases. In: Chemical Communications. 2010 ; Vol. 46, No. 33. pp. 6066-6068.
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