Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

Kam Lau; Vireak Thon; Hai Yu; Li Ding; Yi Chen; Musleh M. Muthana; Denton Wong; Ronald Huang; Xi Chen

doi:10.1039/c0cc01381a

Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

Kam Lau, Vireak Thon, Hai Yu, Li Ding, Yi Chen, Musleh M. Muthana, Denton Wong, Ronald Huang, Xi Chen

Chemistry

Research output: Contribution to journal › Article

70 Scopus citations

Abstract

Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.

Original language	English (US)
Pages (from-to)	6066-6068
Number of pages	3
Journal	Chemical Communications
Volume	46
Issue number	33
DOIs	https://doi.org/10.1039/c0cc01381a
State	Published - Sep 7 2010

ASJC Scopus subject areas

Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Catalysis
Chemistry(all)

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title = "Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases",

abstract = "Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.",

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language = "English (US)",

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T1 - Highly efficient chemoenzymatic synthesis of β1-4-linked galactosides with promiscuous bacterial β1-4-galactosyltransferases

AU - Lau, Kam

AU - Thon, Vireak

AU - Yu, Hai

AU - Ding, Li

AU - Chen, Yi

AU - Muthana, Musleh M.

AU - Wong, Denton

AU - Huang, Ronald

AU - Chen, Xi

PY - 2010/9/7

Y1 - 2010/9/7

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AB - Two bacterial β1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.

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