Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides

Hai Yu, Vireak Thon, Kam Lau, Li Cai, Yi Chen, Shengmao Mu, Yanhong Li, Peng George Wang, Xi Chen

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A novel d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Galβ1-3GalNAc), lacto-N-biose (Galβ1-3GlcNAc), and their derivatives.

Original languageEnglish (US)
Pages (from-to)7507-7509
Number of pages3
JournalChemical Communications
Volume46
Issue number40
DOIs
StatePublished - Oct 28 2010

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Galactokinase
Hexosamines
Galactosides
Phosphorylases
Viral Tumor Antigens
Antigens
Escherichia coli
Enzymes
Derivatives

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Cite this

Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides. / Yu, Hai; Thon, Vireak; Lau, Kam; Cai, Li; Chen, Yi; Mu, Shengmao; Li, Yanhong; Wang, Peng George; Chen, Xi.

In: Chemical Communications, Vol. 46, No. 40, 28.10.2010, p. 7507-7509.

Research output: Contribution to journalArticle

Yu, H, Thon, V, Lau, K, Cai, L, Chen, Y, Mu, S, Li, Y, Wang, PG & Chen, X 2010, 'Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides', Chemical Communications, vol. 46, no. 40, pp. 7507-7509. https://doi.org/10.1039/c0cc02850a
Yu, Hai ; Thon, Vireak ; Lau, Kam ; Cai, Li ; Chen, Yi ; Mu, Shengmao ; Li, Yanhong ; Wang, Peng George ; Chen, Xi. / Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides. In: Chemical Communications. 2010 ; Vol. 46, No. 40. pp. 7507-7509.
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