High variability responses in the hypercholesterolemic hamster model is related to intestinal mucosa acyl coenzyme a: Cholesterol acyltransferase (ACAT) enzyme activities

P. A. Daudu, B. J. German

Research output: Contribution to journalArticle

Abstract

The hypercholesterolernic hamster model is often used to study effects of total dietary cholesterol, fat and fatty acid saturation on plasma lipoprotein cholesterol levels. This study was conducted to determine whether differences in absorption and/or esterification of dietary cholesterol may play a role in widely varying responses in often observed in hamsters fed hypercholesterolemic diets. The relationship between dietary cholesterol esterification and absorption to plasma cholesterol levels was examined by feeding male golden Syrian hamsters diets containing 0, 0.15 and 0.3% cholesterol and high fat. Intestinal acyl coenzyme A:cholesterol acyltransferase (ACAT, the enzyme that eaterifiea cholesterol to fatty acids prior to packaging into chylomicrons) was measured. Animals with high plasma cholesterol, average+SEM;364+27 mG/dL, also showed high ACAT activity {P=0.017) compared to animals with low cholesterol, average+SEM=261+30 mG/dL. The results indicate that high ACAT activitity correlates with high plasma total cholesterol in high fat/high cholesterol diet. This explains extreme variabilities in plasma cholesterol often found in this hamster model. Dietary modification or ACAT inhibition may be useful to lower plasma cholesterol in hypercholesterolemic individuals.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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