High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses

Yanhong Li, Hongzhi Cao, Nguyet Dao, Zheng Luo, Hai Yu, Yi Chen, Zheng Xing, Nicole Baumgarth, Carol Cardona, Xi Chen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Despite the importance of neuraminidase (NA) activity in effective infection by influenza A viruses, limited information exists about the differences of substrate preferences of viral neuraminidases from different hosts or from different strains. Using a high-throughput screening format and a library of twenty α2-3- or α2-6-linked para-nitrophenol-tagged sialylgalactosides, substrate specificity of NAs on thirty-seven strains of human and avian influenza A viruses was studied using intact viral particles. Neuraminidases of all viruses tested cleaved both α2-3- and α2-6-linked sialosides but preferred α2-3-linked ones and the activity was dependent on the terminal sialic acid structure. In contrast to NAs of other subtypes of influenza A viruses which did not cleave 2-keto-3-deoxy-d-glycero- d-galacto-nonulosonic acid (Kdn) or 5-deoxy Kdn (5d-Kdn), NAs of all N7 subtype viruses tested had noticeable hydrolytic activities on α2-3-linked sialosides containing Kdn or 5d-Kdn. Additionally, group 1 NAs showed efficient activity in cleaving N-azidoacetylneuraminic acid from α2-3-linked sialoside.

Original languageEnglish (US)
Pages (from-to)12-19
Number of pages8
JournalVirology
Volume415
Issue number1
DOIs
StatePublished - Jun 20 2011

Fingerprint

Influenza in Birds
Influenza A virus
Neuraminidase
Substrate Specificity
Human Influenza
Viruses
Nitrophenols
Acids
N-Acetylneuraminic Acid
Virion
Libraries
Infection

Keywords

  • Carbohydrate
  • Influenza A virus
  • Inhibitors
  • Neuraminidase
  • Sialic acid
  • Sialosides
  • Substrate specificity studies

ASJC Scopus subject areas

  • Virology

Cite this

High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses. / Li, Yanhong; Cao, Hongzhi; Dao, Nguyet; Luo, Zheng; Yu, Hai; Chen, Yi; Xing, Zheng; Baumgarth, Nicole; Cardona, Carol; Chen, Xi.

In: Virology, Vol. 415, No. 1, 20.06.2011, p. 12-19.

Research output: Contribution to journalArticle

Li, Yanhong ; Cao, Hongzhi ; Dao, Nguyet ; Luo, Zheng ; Yu, Hai ; Chen, Yi ; Xing, Zheng ; Baumgarth, Nicole ; Cardona, Carol ; Chen, Xi. / High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses. In: Virology. 2011 ; Vol. 415, No. 1. pp. 12-19.
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