High throughput in vitro and in vivo screening of inland waters of Southern California

A. C. Mehinto, D. R. Vandervort, W. Lao, G. He, M. S. Denison, S. M. Vliet, D. C. Volz, R. D. Mazor, K. A. Maruya

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2 Scopus citations

Abstract

The impact of unmonitored contaminants, also known as contaminants of emerging concern (CECs), on freshwater streams remains largely uncharacterized. Water samples from 31 streams representing urban, agricultural and undeveloped (i.e., open space) land use in Southern California (USA) were analyzed for in vitro and in vivo bioactivity. The extent and magnitude of bioactivity screened using endocrine-responsive cell bioassays and a fish embryo screening assay were low. In contrast, a wider gradient of responses for the aryl hydrocarbon receptor (AhR) assay was observed, which was negatively correlated with a measure of benthic community structure. Both aromatic and non-aromatic CECs were tentatively identified in these samples, but polycyclic aromatic hydrocarbons (PAHs), known AhR agonists in urban environments, were not present at detectable levels. These results suggest that a combination of in vitro and in vivo show potential as screening techniques for biological condition in situ, but that more advanced, comprehensive analytical methods are needed to identify bioactive contaminants.

Original languageEnglish (US)
Pages (from-to)1142-1149
Number of pages8
JournalEnvironmental Science: Processes and Impacts
Volume19
Issue number9
DOIs
StatePublished - Sep 1 2017

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ASJC Scopus subject areas

  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Management, Monitoring, Policy and Law

Cite this

Mehinto, A. C., Vandervort, D. R., Lao, W., He, G., Denison, M. S., Vliet, S. M., Volz, D. C., Mazor, R. D., & Maruya, K. A. (2017). High throughput in vitro and in vivo screening of inland waters of Southern California. Environmental Science: Processes and Impacts, 19(9), 1142-1149. https://doi.org/10.1039/c7em00170c