Abstract
During primary simian immunodeficiency virus (SIV) infection, CD4+ T cells are severely depleted in gut-associated lymphoid tissue (GALT), while CD8+ T-cell numbers dramatically increase. To gain an understanding of the molecular basis of this disruption in T-cell homeostasis, host gene expression was monitored in longitudinal jejunum tissue biopsies from SIV-infected rhesus macaques by DNA microarray analysis. Transcription of cyclin E1, CDC2, retinoblastoma, transforming growth factor (TGF), fibroblast growth factor (FGF), and interleukin-2 was repressed while cyclins B1 and D2 and transcription factor E2F were upregulated, indicating a complex dysregulation of growth and proliferation within the intestinal mucosa. Innate, cell-mediated, and humoral immune responses were markedly upregulated in animals that significantly reduced their viral loads and retained more intestinal CD4+ T cells. We conclude that the alterations in intestinal gene expression during primary SIV infection were characteristic of a broad-range immune response, and reflective of the efficacy of viral suppression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 84-94 |
| Number of pages | 11 |
| Journal | Virology |
| Volume | 312 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 20 2003 |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection. / George, Michael D.; Sankaran-Walters, Sumathi; Reay, Elizabeth; Gelli, Angela C; Dandekar, Satya.
In: Virology, Vol. 312, No. 1, 20.07.2003, p. 84-94.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection
AU - George, Michael D.
AU - Sankaran-Walters, Sumathi
AU - Reay, Elizabeth
AU - Gelli, Angela C
AU - Dandekar, Satya
PY - 2003/7/20
Y1 - 2003/7/20
N2 - During primary simian immunodeficiency virus (SIV) infection, CD4+ T cells are severely depleted in gut-associated lymphoid tissue (GALT), while CD8+ T-cell numbers dramatically increase. To gain an understanding of the molecular basis of this disruption in T-cell homeostasis, host gene expression was monitored in longitudinal jejunum tissue biopsies from SIV-infected rhesus macaques by DNA microarray analysis. Transcription of cyclin E1, CDC2, retinoblastoma, transforming growth factor (TGF), fibroblast growth factor (FGF), and interleukin-2 was repressed while cyclins B1 and D2 and transcription factor E2F were upregulated, indicating a complex dysregulation of growth and proliferation within the intestinal mucosa. Innate, cell-mediated, and humoral immune responses were markedly upregulated in animals that significantly reduced their viral loads and retained more intestinal CD4+ T cells. We conclude that the alterations in intestinal gene expression during primary SIV infection were characteristic of a broad-range immune response, and reflective of the efficacy of viral suppression.
AB - During primary simian immunodeficiency virus (SIV) infection, CD4+ T cells are severely depleted in gut-associated lymphoid tissue (GALT), while CD8+ T-cell numbers dramatically increase. To gain an understanding of the molecular basis of this disruption in T-cell homeostasis, host gene expression was monitored in longitudinal jejunum tissue biopsies from SIV-infected rhesus macaques by DNA microarray analysis. Transcription of cyclin E1, CDC2, retinoblastoma, transforming growth factor (TGF), fibroblast growth factor (FGF), and interleukin-2 was repressed while cyclins B1 and D2 and transcription factor E2F were upregulated, indicating a complex dysregulation of growth and proliferation within the intestinal mucosa. Innate, cell-mediated, and humoral immune responses were markedly upregulated in animals that significantly reduced their viral loads and retained more intestinal CD4+ T cells. We conclude that the alterations in intestinal gene expression during primary SIV infection were characteristic of a broad-range immune response, and reflective of the efficacy of viral suppression.
UR - http://www.scopus.com/inward/record.url?scp=0043169683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0043169683&partnerID=8YFLogxK
U2 - 10.1016/S0042-6822(03)00207-1
DO - 10.1016/S0042-6822(03)00207-1
M3 - Article
C2 - 12890623
AN - SCOPUS:0043169683
VL - 312
SP - 84
EP - 94
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -