High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels

Charlotte E. Vorwald, Steve S. Ho, Jacklyn Whitehead, Jonathan K Leach

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are a promising cell source for tissue repair and regeneration due to their multilineage capacity, potential for autologous use, and secretion of potent bioactive factors to catalyze the endogenous repair program. However, a major limitation to current cell-based tissue engineering approaches is the drastic loss of cells upon transplantation. The causation of this loss, whether due to apoptosis following a dramatic change in the microenvironment or migration away from the defect site, has yet to be determined. MSCs formed into aggregates, known as spheroids, possess a strong therapeutic advantage compared to the more commonly used dissociated cells due to their improved resistance to apoptosis and increased secretion of endogenous trophic factors. Furthermore, the use of biomaterials such as alginate hydrogels to transplant cells in situ improves cell survival, localizes payloads at the defect site, and facilitates continued instruction of cells by manipulating the biophysical properties of the biomaterial. Transplantation of MSC spheroids without a vehicle into tissue defects comprises the majority of studies to date, ceding control of spheroid function due to the cell’s interaction with the native tissue extracellular matrix and abrogating the established benefits of spheroid formation. Thus, there is a significant need to consider the role of biomaterials in transplanting MSC spheroids using an appropriate carrier. In this chapter, we describe high-throughput formation of spheroids, steps for further characterization, and encapsulation in alginate hydrogels with an eye toward localizing MSC spheroids at the target site.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-149
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1758
ISSN (Print)1064-3745

Fingerprint

Hydrogels
Mesenchymal Stromal Cells
Biocompatible Materials
Mesenchymal Stem Cell Transplantation
Apoptosis
Cell Transplantation
Tissue Engineering
Cell Communication
Causality
Extracellular Matrix
Regeneration
Cell Survival
Transplants
alginic acid
Therapeutics

Keywords

  • Alginate
  • Encapsulation
  • Mesenchymal stem cell
  • Spheroid
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Vorwald, C. E., Ho, S. S., Whitehead, J., & Leach, J. K. (2018). High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels. In Methods in Molecular Biology (pp. 139-149). (Methods in Molecular Biology; Vol. 1758). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7741-3_11

High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels. / Vorwald, Charlotte E.; Ho, Steve S.; Whitehead, Jacklyn; Leach, Jonathan K.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 139-149 (Methods in Molecular Biology; Vol. 1758).

Research output: Chapter in Book/Report/Conference proceedingChapter

Vorwald, CE, Ho, SS, Whitehead, J & Leach, JK 2018, High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1758, Humana Press Inc., pp. 139-149. https://doi.org/10.1007/978-1-4939-7741-3_11
Vorwald CE, Ho SS, Whitehead J, Leach JK. High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 139-149. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7741-3_11
Vorwald, Charlotte E. ; Ho, Steve S. ; Whitehead, Jacklyn ; Leach, Jonathan K. / High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 139-149 (Methods in Molecular Biology).
@inbook{2bc768d87d804152b7f090601bd23834,
title = "High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels",
abstract = "Mesenchymal stem cells (MSCs) are a promising cell source for tissue repair and regeneration due to their multilineage capacity, potential for autologous use, and secretion of potent bioactive factors to catalyze the endogenous repair program. However, a major limitation to current cell-based tissue engineering approaches is the drastic loss of cells upon transplantation. The causation of this loss, whether due to apoptosis following a dramatic change in the microenvironment or migration away from the defect site, has yet to be determined. MSCs formed into aggregates, known as spheroids, possess a strong therapeutic advantage compared to the more commonly used dissociated cells due to their improved resistance to apoptosis and increased secretion of endogenous trophic factors. Furthermore, the use of biomaterials such as alginate hydrogels to transplant cells in situ improves cell survival, localizes payloads at the defect site, and facilitates continued instruction of cells by manipulating the biophysical properties of the biomaterial. Transplantation of MSC spheroids without a vehicle into tissue defects comprises the majority of studies to date, ceding control of spheroid function due to the cell’s interaction with the native tissue extracellular matrix and abrogating the established benefits of spheroid formation. Thus, there is a significant need to consider the role of biomaterials in transplanting MSC spheroids using an appropriate carrier. In this chapter, we describe high-throughput formation of spheroids, steps for further characterization, and encapsulation in alginate hydrogels with an eye toward localizing MSC spheroids at the target site.",
keywords = "Alginate, Encapsulation, Mesenchymal stem cell, Spheroid, Transplantation",
author = "Vorwald, {Charlotte E.} and Ho, {Steve S.} and Jacklyn Whitehead and Leach, {Jonathan K}",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-7741-3_11",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "139--149",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels

AU - Vorwald, Charlotte E.

AU - Ho, Steve S.

AU - Whitehead, Jacklyn

AU - Leach, Jonathan K

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Mesenchymal stem cells (MSCs) are a promising cell source for tissue repair and regeneration due to their multilineage capacity, potential for autologous use, and secretion of potent bioactive factors to catalyze the endogenous repair program. However, a major limitation to current cell-based tissue engineering approaches is the drastic loss of cells upon transplantation. The causation of this loss, whether due to apoptosis following a dramatic change in the microenvironment or migration away from the defect site, has yet to be determined. MSCs formed into aggregates, known as spheroids, possess a strong therapeutic advantage compared to the more commonly used dissociated cells due to their improved resistance to apoptosis and increased secretion of endogenous trophic factors. Furthermore, the use of biomaterials such as alginate hydrogels to transplant cells in situ improves cell survival, localizes payloads at the defect site, and facilitates continued instruction of cells by manipulating the biophysical properties of the biomaterial. Transplantation of MSC spheroids without a vehicle into tissue defects comprises the majority of studies to date, ceding control of spheroid function due to the cell’s interaction with the native tissue extracellular matrix and abrogating the established benefits of spheroid formation. Thus, there is a significant need to consider the role of biomaterials in transplanting MSC spheroids using an appropriate carrier. In this chapter, we describe high-throughput formation of spheroids, steps for further characterization, and encapsulation in alginate hydrogels with an eye toward localizing MSC spheroids at the target site.

AB - Mesenchymal stem cells (MSCs) are a promising cell source for tissue repair and regeneration due to their multilineage capacity, potential for autologous use, and secretion of potent bioactive factors to catalyze the endogenous repair program. However, a major limitation to current cell-based tissue engineering approaches is the drastic loss of cells upon transplantation. The causation of this loss, whether due to apoptosis following a dramatic change in the microenvironment or migration away from the defect site, has yet to be determined. MSCs formed into aggregates, known as spheroids, possess a strong therapeutic advantage compared to the more commonly used dissociated cells due to their improved resistance to apoptosis and increased secretion of endogenous trophic factors. Furthermore, the use of biomaterials such as alginate hydrogels to transplant cells in situ improves cell survival, localizes payloads at the defect site, and facilitates continued instruction of cells by manipulating the biophysical properties of the biomaterial. Transplantation of MSC spheroids without a vehicle into tissue defects comprises the majority of studies to date, ceding control of spheroid function due to the cell’s interaction with the native tissue extracellular matrix and abrogating the established benefits of spheroid formation. Thus, there is a significant need to consider the role of biomaterials in transplanting MSC spheroids using an appropriate carrier. In this chapter, we describe high-throughput formation of spheroids, steps for further characterization, and encapsulation in alginate hydrogels with an eye toward localizing MSC spheroids at the target site.

KW - Alginate

KW - Encapsulation

KW - Mesenchymal stem cell

KW - Spheroid

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=85045908065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045908065&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7741-3_11

DO - 10.1007/978-1-4939-7741-3_11

M3 - Chapter

C2 - 29679328

AN - SCOPUS:85045908065

T3 - Methods in Molecular Biology

SP - 139

EP - 149

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -