High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems

Conary Meyer, Chuqing Zhou, Zecong Fang, Marjorie L. Longo, Tingrui Pan, Cheemeng Tan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-134
Number of pages14
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2433
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Automation
  • Cell-free protein synthesis
  • Droplet printing
  • High throughput
  • Low-volume liquid handling
  • Microfluidic adaptive printing
  • Protein screening

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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