@inbook{2772f4124d55425ab0b8d126e44cbe19,
title = "High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems",
abstract = "Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.",
keywords = "Automation, Cell-free protein synthesis, Droplet printing, High throughput, Low-volume liquid handling, Microfluidic adaptive printing, Protein screening",
author = "Conary Meyer and Chuqing Zhou and Zecong Fang and Longo, {Marjorie L.} and Tingrui Pan and Cheemeng Tan",
note = "Funding Information: This work was partially supported by the National Science Foundation under Grant No. DMR?1806366 and the NIEHS UC Davis Superfund Project 5P42ES004699-30. The research was also partially supported by the ARCS fellowship to C. M. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1998-8_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "121--134",
booktitle = "Methods in Molecular Biology",
}