High-throughput dose-response measurement using a label-free microarray-in-microplate assay platform

J. P. Landry, G. Malovichko, X. D. Zhu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Microarray-based binding assays facilitate the discovery of protein ligands from large collections of small molecules. Hundreds of ligands can be identified, yet only a small portion of them have interfering effects (competitive or noncompetitive) on a specific protein-receptor binding reaction. Further efficient screening of ligands for those with specific modifying effect is needed in order to take the full advantage of throughputs of microarray-based assays for drug discovery. We report a label-free "microarray-in-microplate" assay platform for simultaneous acquisition of at least 32 dose-response curves in a single experiment, each curve having 12 concentration points. When combined with ligand discovery, this makes the microarray-based platform a true high-throughout means of finding inhibitors to specific protein-receptor reactions starting from a large collection of small-molecule libraries.

Original languageEnglish (US)
Pages (from-to)5640-5648
Number of pages9
JournalAnalytical Chemistry
Volume87
Issue number11
DOIs
StatePublished - Jun 2 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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