High throughput analysis of nuclear receptor-cofactor interactions.

Michael L. Goodson; Behnom Farboud; Martin L. Privalsky

doi:10.1007/978-1-60327-575-0_9

High throughput analysis of nuclear receptor-cofactor interactions.

Michael L. Goodson, Behnom Farboud, Martin L. Privalsky

Microbiology

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

Various assays have been employed to study the nuclear receptor/cofactor interactions. Coimmunoprecipitation protocols, both yeast and mammalian two-hybrid systems, and electrophoretic mobility shift/supershift assays are all commonly used. One of the most useful assays for studying direct protein-protein interactions is the glutathione-S-transferase "pulldown" assay. We have developed a high-throughput version of this assay that utilizes a filter microplate to allow parallel processing of many samples, significantly reducing the time and reagents required for the assay and increasing the sensitivity of the assay for weaker protein-protein interactions.

Original language	English (US)
Pages (from-to)	157-169
Number of pages	13
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	505
DOIs	https://doi.org/10.1007/978-1-60327-575-0_9
State	Published - 2009

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ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Goodson, M. L., Farboud, B., & Privalsky, M. L. (2009). High throughput analysis of nuclear receptor-cofactor interactions. Methods in molecular biology (Clifton, N.J.), 505, 157-169. https://doi.org/10.1007/978-1-60327-575-0_9

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10.1007/978-1-60327-575-0_9