High throughput analysis of nuclear receptor-cofactor interactions.

Michael L. Goodson, Behnom Farboud, Martin L. Privalsky

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Various assays have been employed to study the nuclear receptor/cofactor interactions. Coimmunoprecipitation protocols, both yeast and mammalian two-hybrid systems, and electrophoretic mobility shift/supershift assays are all commonly used. One of the most useful assays for studying direct protein-protein interactions is the glutathione-S-transferase "pulldown" assay. We have developed a high-throughput version of this assay that utilizes a filter microplate to allow parallel processing of many samples, significantly reducing the time and reagents required for the assay and increasing the sensitivity of the assay for weaker protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)157-169
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume505
DOIs
StatePublished - 2009

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Cytoplasmic and Nuclear Receptors
Proteins
Two-Hybrid System Techniques
Electrophoretic Mobility Shift Assay
Glutathione Transferase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

High throughput analysis of nuclear receptor-cofactor interactions. / Goodson, Michael L.; Farboud, Behnom; Privalsky, Martin L.

In: Methods in molecular biology (Clifton, N.J.), Vol. 505, 2009, p. 157-169.

Research output: Contribution to journalArticle

Goodson, Michael L. ; Farboud, Behnom ; Privalsky, Martin L. / High throughput analysis of nuclear receptor-cofactor interactions. In: Methods in molecular biology (Clifton, N.J.). 2009 ; Vol. 505. pp. 157-169.
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