High Tensile Strength of Engineered β-Solenoid Fibrils via Sonication and Pulling

Zeyu Peng, Amanda S. Parker, Maria D.R. Peralta, Krishnakumar M. Ravikumar, Daniel L. Cox, Michael D. Toney

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We present estimates of ultimate tensile strength (UTS) for two engineered β-solenoid protein mutant fibril structures (spruce budworm and Rhagium inquisitor antifreeze proteins) derived from sonication-based measurements and from force pulling molecular dynamics simulations, both in water. Sonication experiments generate limiting scissioned fibrils with a well-defined length-to-width correlation for the mutant spruce budworm protein and the resultant UTS estimate is 0.66 ± 0.08 GPa. For fibrils formed from engineered R. inquisitor antifreeze protein, depending upon geometry, we estimate UTSs of 3.5 ± 3.2–5.5 ± 5.1 GPa for proteins with interfacial disulfide bonds, and 1.6 ± 1.5–2.5 ± 2.3 GPa for the reduced form. The large error bars for the R. inquisitor structures are intrinsic to the broad distribution of limiting scission lengths. Simulations provide pulling velocity-dependent UTSs increasing from 0.2 to 1 GPa in the available speed range, and 1.5 GPa extrapolated to the speeds expected in the sonication experiments. Simulations yield low-velocity values for the Young's modulus of 6.0 GPa. Without protein optimization, these mechanical parameters are similar to those of spider silk and Kevlar, but in contrast to spider silk, these proteins have a precisely known sequence-structure relationship.

Original languageEnglish (US)
Pages (from-to)1945-1955
Number of pages11
JournalBiophysical Journal
Volume113
Issue number9
DOIs
StatePublished - Nov 7 2017

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Sonication
Tensile Strength
Antifreeze Proteins
Silk
Arthropod Proteins
Proteins
Spiders
Elastic Modulus
Mutant Proteins
Molecular Dynamics Simulation
Disulfides
Water

ASJC Scopus subject areas

  • Biophysics

Cite this

Peng, Z., Parker, A. S., Peralta, M. D. R., Ravikumar, K. M., Cox, D. L., & Toney, M. D. (2017). High Tensile Strength of Engineered β-Solenoid Fibrils via Sonication and Pulling. Biophysical Journal, 113(9), 1945-1955. https://doi.org/10.1016/j.bpj.2017.09.003

High Tensile Strength of Engineered β-Solenoid Fibrils via Sonication and Pulling. / Peng, Zeyu; Parker, Amanda S.; Peralta, Maria D.R.; Ravikumar, Krishnakumar M.; Cox, Daniel L.; Toney, Michael D.

In: Biophysical Journal, Vol. 113, No. 9, 07.11.2017, p. 1945-1955.

Research output: Contribution to journalArticle

Peng, Z, Parker, AS, Peralta, MDR, Ravikumar, KM, Cox, DL & Toney, MD 2017, 'High Tensile Strength of Engineered β-Solenoid Fibrils via Sonication and Pulling', Biophysical Journal, vol. 113, no. 9, pp. 1945-1955. https://doi.org/10.1016/j.bpj.2017.09.003
Peng Z, Parker AS, Peralta MDR, Ravikumar KM, Cox DL, Toney MD. High Tensile Strength of Engineered β-Solenoid Fibrils via Sonication and Pulling. Biophysical Journal. 2017 Nov 7;113(9):1945-1955. https://doi.org/10.1016/j.bpj.2017.09.003
Peng, Zeyu ; Parker, Amanda S. ; Peralta, Maria D.R. ; Ravikumar, Krishnakumar M. ; Cox, Daniel L. ; Toney, Michael D. / High Tensile Strength of Engineered β-Solenoid Fibrils via Sonication and Pulling. In: Biophysical Journal. 2017 ; Vol. 113, No. 9. pp. 1945-1955.
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